Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-112   THR-113  12.4 12.4 5.0 8.1 64.0 60.3 -13.7
 THR-113   LYS-114  9.2 9.1 6.4 -19.1 123.8 111.1 18.8
 LYS-114   GLY-115  7.5 6.7 -15.5 -11.2 79.4 71.1 17.0
 GLY-115   PRO-116  4.1 3.4 12.7 1.2 83.1 96.1 4.4
 PRO-116   GLU-117  0.6 0.5 -67.6 28.4 33.5 34.2 78.3
 GLU-117   ILE-118  3.3 3.3 -5.3 5.6 102.5 101.2 -2.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-216   LEU-217  9.4 8.4 67.5 -53.9 129.5 123.9 -15.4
 LEU-217   PRO-218  6.0 4.9 -19.7 1.8 61.7 64.0 17.5
 PRO-218   ALA-219  4.9 3.9 -3.8 -13.3 124.2 117.7 -6.2
 ALA-219   VAL-220  5.7 4.4 3.4 3.7 90.4 115.1 -10.7
 VAL-220   SER-221  3.6 3.3 -59.4 45.2 67.7 60.3 -2.0
 SER-221   GLU-222  1.8 1.7 -6.7 -2.0 31.9 23.0 33.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees