Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 PHE-165   TYR-166  15.9 15.6 15.8 -17.9 79.6 83.2 5.6
 TYR-166   ARG-167  16.5 16.3 3.2 -12.5 38.5 43.1 -15.9
 ARG-167   ASP-168  14.1 14.0 29.5 -14.9 35.7 34.5 11.7
 ASP-168   GLN-169  14.3 13.0 -45.7 24.5 111.2 91.3 -52.8
 GLN-169   PRO-170  10.6 9.6 -26.2 -14.1 36.0 15.2 158.1
 PRO-170   HIS-171  7.8 7.7 12.6 1.1 130.0 122.9 -32.7
 HIS-171   PRO-172  7.0 7.4 -5.0 1.2 110.2 106.9 -24.5
 PRO-172   PHE-173  5.5 6.0 -2.1 0.9 66.9 62.6 31.8
 PHE-173   ILE-174  2.5 3.3 -0.6 0.2 125.9 121.0 -5.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LYS-224   ALA-225  5.3 4.9 -4.0 6.2 113.4 120.8 -10.0
 ALA-225   SER-226  8.8 8.3 -37.5 14.6 109.8 108.0 -78.8
 GLN-229   SER-230  8.1 6.5 -20.7 20.9 41.6 53.4 1.9
 SER-230   ASP-231  7.9 8.6 22.9 40.8 76.9 94.5 -38.9
 ASP-231   ASP-232  8.8 10.3 -61.2 52.1 28.5 27.8 49.8
 ASP-232   LEU-233  8.8 8.3 -16.8 -14.4 76.2 99.9 14.4
 LEU-233   LEU-234  8.1 7.6 13.4 -2.0 75.3 70.6 3.7
 LEU-234   THR-235  4.3 3.9 7.2 -20.4 14.0 7.3 -52.6
 THR-235   HIS-236  4.5 4.1 10.4 4.4 75.1 67.1 45.7
 HIS-236   MET-237  7.5 7.3 3.3 -15.8 113.8 99.9 -2.5
 HIS-236   MET-237  7.5 7.3 3.3 -15.8 113.8 99.9 -2.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 HIS-236   MET-237  7.5 7.3 3.3 -15.8 113.8 99.9 -2.5
 HIS-236   MET-237  7.5 7.3 3.3 -15.8 113.8 99.9 -2.5
 MET-237   LEU-238  6.8 6.8 0.8 20.5 53.3 50.0 31.2
 LEU-238   ASN-239  4.7 4.2 -3.6 16.2 164.5 165.4 40.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees