Ferric-Iron Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-100   ARG-101  4.8 4.9 8.3 -11.7 116.8 114.2 -11.4  ARG-101   SER-102  1.9 2.1 10.6 9.9 41.8 45.1 72.8  SER-102   ARG-103  1.7 1.9 -13.4 10.5 89.4 99.3 -14.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-224   VAL-225  2.1 2.3 28.3 -17.8 30.1 24.7 38.4  VAL-225   THR-226  0.8 0.4 11.8 -24.9 101.4 104.0 20.1  THR-226   TYR-227  4.2 4.2 38.4 -28.9 52.4 42.2 28.4  TYR-227   SER-228  7.0 7.2 -21.5 34.3 108.7 109.1 14.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-268   PRO-269  14.1 14.3 5.1 9.6 105.0 99.8 -29.2  PRO-269   HIS-270  15.0 15.8 -12.1 10.0 26.8 24.0 13.9  HIS-270   VAL-271  16.7 17.2 -14.0 -6.2 43.1 49.0 54.2  VAL-271   VAL-272  13.3 13.7 4.8 -20.2 95.3 77.0 28.1  VAL-272   SER-273  11.4 11.4 16.0 -3.7 111.7 131.1 -31.2  SER-273   THR-274  7.6 7.8 -4.3 -14.0 30.8 49.0 62.0  THR-274   PHE-275  6.1 7.6 14.2 17.6 75.0 90.1 58.3  PHE-275   ASN-276  4.7 4.8 -130.9 121.1 122.0 119.7 -35.1  ASN-276   LEU-277  7.8 7.8 -39.2 53.8 170.5 156.4 58.9  LEU-277   GLU-278  7.3 6.1 7.3 24.3 67.3 50.7 58.1  GLU-278   PRO-279  10.2 8.2 -34.3 -0.2 118.0 122.5 -52.5  PRO-279   ILE-280  11.0 9.9 -12.7 0.0 143.5 155.9 -27.9  ILE-280   ALA-281  8.4 7.9 -12.6 11.4 152.2 145.2 -14.0  ALA-281   LYS-282  10.9 10.7 -19.0 10.1 97.1 95.6 5.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees