Dna-Directed RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-460   VAL-461  15.4 16.8 -3.9 115.7 96.6 145.2 -381.9
 VAL-461   ASP-462  16.9 18.9 -37.5 177.4 151.2 138.5 220.1
 ASP-462   ALA-463  17.1 18.0 92.8 -70.1 48.6 45.5 26.4
 ALA-463   LEU-464  18.1 19.9 -151.0 -37.7 76.6 91.2 -114.1
 LEU-464   GLY-465  18.8 21.0 9.1 94.1 54.5 136.7 90.4
 GLY-465   PHE-466  18.9 20.9 65.3 89.9 129.5 99.0 -139.4
 PHE-466   ILE-467  18.6 17.6 -71.3 -81.8 47.1 43.8 182.6
 ILE-467   ARG-468  16.8 16.2 -0.7 -94.8 58.8 102.4 35.0
 ARG-468   THR-469  15.4 12.8 -157.6 -172.2 98.2 37.1 -15.4
 THR-469   PRO-470  11.8 10.0 43.6 6.6 138.4 73.9 -14.9
 PRO-470   TYR-471  9.9 8.1 -53.7 -42.4 110.3 88.8 -6.5
 TYR-471   ARG-472  8.5 4.8 -27.8 60.4 87.7 114.0 -165.4
 ARG-472   ARG-473  5.6 5.9 20.8 -6.5 70.9 59.6 22.5
 ARG-473   VAL-474  6.9 5.8 -22.1 22.1 138.4 69.1 -11.1
 VAL-474   LYS-475  7.2 7.4 40.7 -152.7 87.7 39.3 219.2
 LYS-475   ASN-476  10.5 9.3 31.5 11.6 93.8 109.3 -80.1
 ASN-476   GLY-477  12.6 11.6 -126.0 -50.9 128.8 6.1 -146.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-530   PHE-531  3.2 5.3 -66.1 -86.9 164.3 116.3 -224.3
 PHE-531   MET-532  3.0 2.6 -12.3 149.4 132.3 171.8 219.9
 MET-532   ASP-533  3.5 2.5 11.2 47.0 83.4 72.4 27.6
 ASP-533   VAL-534  3.8 2.1 14.1 -6.2 55.6 65.9 -1.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees