Dnab Replication Fork Helicase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-145   GLU-146  15.1 14.8 9.4 -4.0 87.1 89.2 0.2
 GLU-146   VAL-147  15.1 14.6 -14.5 -11.3 63.5 56.0 13.9
 VAL-147   ALA-148  18.6 18.0 -2.4 28.6 16.7 15.9 -32.1
 ALA-148   LEU-149  17.2 17.2 9.6 -17.9 98.3 127.0 -5.3
 ALA-156   ARG-157  19.6 6.1 39.8 -78.3 143.3 132.4 37.4
 ARG-157   PRO-158  20.7 8.6 -17.3 8.5 84.1 98.0 -0.3
 PRO-158   MET-159  24.0 12.0 10.0 -13.9 142.0 133.9 4.0
 MET-159   ARG-160  24.1 13.6 2.2 11.0 152.7 129.1 -13.6
 ARG-160   GLU-161  26.3 16.5 -8.4 -13.2 58.3 26.9 26.0
 GLU-161   LEU-162  23.8 14.6 19.8 -4.7 111.4 122.5 -13.2
 LEU-162   VAL-163  21.0 12.3 4.7 -11.2 141.9 122.6 4.7
 VAL-163   HIS-164  23.1 15.6 3.8 -4.3 148.7 136.0 1.1
 HIS-164   GLU-165  23.7 16.8 -4.2 12.8 63.1 35.5 -12.2
 GLU-165   THR-166  20.0 13.7 -7.9 7.8 69.5 59.7 -0.5
 THR-166   PHE-167  18.9 13.7 -14.0 14.1 31.4 56.9 4.1
 PHE-167   GLU-168  21.7 17.4 -6.0 -4.6 40.2 30.1 15.7
 GLU-168   HIS-169  20.7 17.0 4.9 6.3 114.3 135.0 -12.3
 HIS-169   ILE-170  17.1 13.9 0.7 17.2 123.2 118.9 -20.8
 ILE-170   GLU-171  18.5 16.4 -45.3 30.5 31.3 43.1 17.7
 GLU-171   ALA-172  20.2 18.7 0.7 10.1 130.3 147.6 -9.4
 VAL-183   ARG-184  4.5 4.9 1.6 3.8 92.3 92.5 6.2
 ARG-184   THR-185  7.2 7.3 7.1 1.0 94.9 100.9 -4.5

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees