Pyruvate Kinase Isozymes M1/m2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  9.5 9.4 -5.3 5.1 56.1 59.0 5.9
 LYS-114   GLY-115  8.3 8.2 -8.6 4.6 75.1 72.2 -18.5
 GLY-115   PRO-116  4.5 4.4 16.4 0.1 87.2 95.3 -18.7
 PRO-116   GLU-117  3.3 3.0 -43.5 26.6 31.8 47.0 117.9
 GLU-117   ILE-118  6.2 6.4 -22.6 -0.5 98.3 95.4 30.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-218   ALA-219  3.6 3.5 13.0 -5.3 62.2 62.9 44.1
 ALA-219   VAL-220  3.4 3.3 -6.2 14.4 75.3 68.4 23.2
 VAL-220   SER-221  2.4 2.5 19.6 -34.0 119.6 117.0 63.4
 SER-221   GLU-222  2.7 2.2 -6.8 0.1 26.6 34.0 16.2
 GLU-222   LYS-223  4.5 3.9 5.9 -1.4 86.2 78.9 -27.6
 LYS-223   ASP-224  7.5 7.2 -3.8 -6.9 100.2 108.5 -2.8
 ASP-224   ILE-225  6.5 6.5 8.3 -6.6 144.7 138.9 21.3
 ILE-225   GLN-226  6.0 6.2 7.9 -6.0 114.6 116.7 -35.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees