Biotin Ligase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 SER-216   LEU-217  4.0 3.7 -4.4 -3.0 58.3 58.7 55.2
 LEU-217   THR-218  2.1 2.0 1.8 4.9 45.2 46.4 7.9
 THR-218   ILE-219  2.6 2.6 5.0 -0.4 60.6 60.1 33.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 THR-254   VAL-255  8.6 8.9 1.6 0.1 103.2 109.8 16.6
 VAL-255   VAL-256  7.0 7.1 7.4 -4.8 63.4 63.8 5.0
 VAL-256   VAL-257  3.5 3.5 -0.7 -6.9 96.4 93.9 15.4
 VAL-257   SER-258  5.0 5.1 1.0 -8.2 80.1 75.2 -35.8
 SER-258   ALA-259  6.3 6.4 4.7 5.4 23.7 30.5 99.6
 ALA-259   GLY-260  7.8 8.2 0.1 2.3 76.8 83.9 -57.5
 GLY-260   ASP-261  9.9 10.3 8.8 4.5 36.2 38.9 68.4
 ASP-261   VAL-262  10.0 11.1 -20.0 2.3 101.7 93.1 52.1
 ASP-261   VAL-262  10.0 11.1 -20.0 2.3 101.7 93.1 52.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ASP-261   VAL-262  10.0 11.1 -20.0 2.3 101.7 93.1 52.1
 ASP-261   VAL-262  10.0 11.1 -20.0 2.3 101.7 93.1 52.1
 VAL-262   VAL-263  8.4 9.2 3.3 -11.7 157.5 164.1 39.5
 VAL-263   HIS-264  9.8 10.3 5.7 0.6 104.9 102.0 -32.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees