Fab 4E10

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-105   VAL-106  5.0 4.8 -8.6 7.0 39.1 37.0 -0.1
 VAL-106   LYS-107  1.8 1.8 -9.4 9.4 87.3 85.4 7.8
 LYS-107   ARG-108  3.3 2.7 11.2 -14.0 104.5 106.9 3.4
 ARG-108   THR-109  4.8 4.3 4.4 -5.8 87.8 86.6 3.3
 THR-109   VAL-110  8.6 8.1 12.7 -8.4 24.2 21.3 35.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLN-166   ASP-167  1.7 1.9 -4.1 2.9 94.5 93.2 -17.2
 ASP-167   SER-168  4.5 5.0 0.7 2.2 30.7 33.3 13.8
 SER-168   LYS-169  7.0 7.5 -6.2 17.7 129.1 129.9 78.4
 LYS-169   ASP-170  8.5 9.0 -18.6 5.9 106.7 116.2 -55.6
 ASP-170   SER-171  7.2 7.3 2.3 2.8 83.5 87.3 -12.5
 SER-171   THR-172  3.4 3.6 -1.1 3.9 58.5 60.6 1.2
 THR-172   TYR-173  4.2 4.2 5.6 1.4 145.2 147.8 -25.9
 TYR-173   SER-174  3.2 3.2 5.9 -4.6 137.5 135.5 -18.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees