Antibody 7A1 Fab'

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-114   LYS-115  7.6 7.4 1.0 -7.0 66.8 63.8 -47.2
 LYS-115   THR-116  5.1 4.6 14.6 -10.7 126.4 129.0 -8.2
 THR-116   THR-117  2.3 2.0 4.6 4.7 87.6 87.8 16.3
 THR-117   PRO-118  5.6 5.4 -3.0 1.1 52.9 54.1 49.8

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees