Chimeric 48G7 Fab

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-108   LEU-109  4.2 4.3 14.9 -12.2 149.1 150.7 -7.2
 LEU-109   GLU-110  3.2 3.3 10.5 6.2 77.1 78.0 6.8
 GLU-110   ILE-111  1.3 1.7 -46.3 16.4 31.8 33.3 70.5

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees