Metabotropic Glutamate Receptor 2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-185   PRO-186  4.7 5.0 -19.1 -2.0 42.4 39.3 68.9  PRO-186   PRO-187  2.0 2.4 -6.2 12.7 33.4 49.0 -43.5  PRO-187   ASP-188  1.1 1.2 -4.4 6.4 91.9 101.9 15.6  ASP-188   PHE-189  3.1 3.6 -17.1 -6.8 66.9 62.3 48.6  PHE-189   PHE-190  4.2 3.9 29.3 -11.7 37.1 52.5 29.3  PHE-190   GLN-191  5.3 4.8 9.3 -11.9 69.0 61.3 6.0  GLN-191   ALA-192  6.3 6.8 28.2 -8.5 125.2 125.6 -16.6  ALA-192   LYS-193  8.4 8.9 -3.0 9.4 95.6 95.4 14.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-319   ALA-320  4.2 4.2 -12.1 -24.8 13.3 13.3 131.7  ALA-320   SER-321  2.6 2.7 -9.5 32.9 63.2 71.5 -34.3  SER-321   TYR-322  3.1 3.1 1.9 -9.7 161.9 172.2 27.5  TYR-322   PRO-323  4.2 4.0 6.6 -8.9 99.5 101.2 -7.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-447   ASP-448  10.8 10.7 -6.6 25.2 114.6 115.0 30.7  ASP-448   GLY-449  7.2 7.0 -9.5 14.0 159.4 163.4 32.8  GLY-449   ILE-450  5.7 6.3 -23.1 17.1 87.7 98.3 -21.7  ILE-450   GLY-451  4.8 6.5 -24.5 -1.6 92.1 106.8 -30.8  GLY-451   ARG-452  3.6 3.9 24.9 1.0 36.6 44.4 50.0  ARG-452   TYR-453  3.8 4.1 -8.5 10.7 98.0 99.5 43.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees