Influenza Recombinant Ha2 Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-120   LYS-121  8.4 8.0 -0.8 3.8 57.9 58.5 -7.7  LYS-121   THR-122  7.6 8.0 -9.1 12.3 23.4 28.5 -2.7  THR-122   ARG-123  7.9 7.5 -2.4 -1.3 92.8 97.5 -7.4  ARG-123   ARG-124  7.0 5.3 1.6 -2.4 85.8 84.0 -8.2  ARG-124   GLN-125  3.4 2.8 -16.6 5.8 34.2 35.0 12.2  GLN-125   LEU-126  2.5 4.1 3.0 -19.1 124.2 132.4 12.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-143   CYS-144  6.0 5.0 -0.3 -6.3 147.3 144.8 -3.7  CYS-144   ASP-145  6.0 5.3 6.9 -2.0 36.9 39.8 2.3  ASP-145   ASN-146  5.7 4.3 7.2 -2.8 37.4 43.7 19.3  ASN-146   ALA-147  4.8 3.4 171.3 -161.0 81.4 67.9 2.2  ALA-147   CYS-148  8.2 7.0 11.5 4.2 137.1 129.7 -24.8  CYS-148   ILE-149  9.0 8.2 -8.6 12.5 96.7 95.7 -6.8  ILE-149   GLU-150  7.1 7.0 -3.8 8.2 145.3 143.3 6.4  GLU-150   SER-151  8.5 8.3 -4.4 -6.3 81.1 82.2 5.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees