Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-85   GLY-86  9.3 8.8 120.4 -126.0 111.7 91.5 -36.7  GLY-86   PRO-87  6.3 5.8 30.6 -12.5 103.5 108.3 -44.5  PRO-87   GLU-88  7.7 7.3 -71.3 34.3 25.4 23.7 206.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-184   ASP-185  9.4 10.6 -0.1 35.9 20.9 26.2 -199.7  ASP-185   LEU-186  10.5 12.1 6.4 -43.1 140.2 135.4 205.3  LEU-186   PRO-187  9.5 9.8 -4.0 -22.8 45.0 59.2 99.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees