Calg1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-51   ALA-52  7.8 7.9 -2.2 3.1 36.7 37.7 -28.2  ALA-52   THR-53  6.4 6.3 1.6 -10.2 123.2 120.5 13.5  THR-53   GLY-54  4.2 4.0 3.5 -27.5 143.9 148.6 166.4  PRO-56   VAL-57  9.2 9.1 -5.2 -1.6 18.1 26.2 55.1  VAL-57   PHE-58  9.0 8.3 -1.6 8.2 72.6 70.8 -21.2  PHE-58   ASP-59  11.1 10.8 -2.1 -3.1 103.2 109.0 -38.2  ASP-59   GLY-60  9.4 9.7 3.3 -2.7 123.0 114.2 1.8  GLY-60   PHE-61  6.3 6.3 10.0 -2.0 152.9 152.7 -54.8  PHE-61   LEU-62  8.0 8.0 5.4 -4.1 86.6 85.3 8.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-87   ILE-88  1.5 1.4 -0.7 3.3 39.2 39.6 -23.2  ILE-88   VAL-89  3.5 3.4 1.3 -6.0 146.1 146.1 49.9  VAL-89   PHE-90  2.0 2.1 -1.2 -12.2 66.6 61.8 32.5  PHE-90   GLY-91  1.8 1.9 14.0 -0.7 97.6 90.0 -34.5  GLY-91   ARG-92  4.4 3.9 -6.3 -6.0 54.6 52.2 51.2  ARG-92   VAL-93  5.0 4.7 -5.0 14.2 35.8 38.4 -59.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees