Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-171   PRO-172  4.4 3.8 -1.5 10.6 88.6 81.3 -25.2
 PRO-172   PHE-173  4.2 3.2 -9.5 -8.0 63.7 70.3 23.9
 PHE-173   ILE-174  1.3 0.8 7.4 -1.9 48.0 42.2 23.3
 ILE-174   THR-175  1.2 2.0 3.2 4.3 52.5 53.2 13.8
 THR-175   SER-176  2.9 2.5 -3.6 -6.3 63.5 68.7 15.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-213   ASP-214  5.8 5.9 11.8 -11.0 66.4 68.9 9.8
 ASP-214   LEU-215  5.4 5.3 3.6 -3.0 90.2 87.1 12.9
 LEU-215   VAL-216  2.1 1.8 2.1 5.0 42.3 35.3 23.0
 VAL-216   ASP-217  1.5 2.0 7.5 -9.1 28.4 36.0 2.4
 ASP-217   LYS-218  2.6 2.8 4.2 5.8 81.4 83.6 7.9
 LYS-218   ILE-219  3.6 3.5 1.0 -6.9 80.1 70.3 18.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees