Calmodulin

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-15   PHE-16  6.6 6.2 -1.6 -7.6 111.4 115.8 -2.1  PHE-16   SER-17  5.7 5.4 -0.1 10.3 147.2 142.5 4.7  SER-17   LEU-18  7.0 6.8 -12.9 3.4 82.2 78.8 -3.8  LEU-18   PHE-19  10.2 10.1 -6.5 0.3 92.8 88.7 0.8  LEU-18   PHE-19  10.2 10.1 -6.5 0.3 92.8 88.7 0.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-18   PHE-19  10.2 10.1 -6.5 0.3 92.8 88.7 0.8  LEU-18   PHE-19  10.2 10.1 -6.5 0.3 92.8 88.7 0.8  PHE-19   ASP-20  11.2 10.9 3.0 5.2 149.4 148.8 -4.4  ASP-20   LYS-21  9.8 9.7 8.1 13.6 141.2 140.7 -11.7  LYS-21   ASP-22  11.6 12.0 -25.2 3.3 21.6 19.1 13.3  ASP-22   GLY-23  11.3 11.3 1.9 -5.0 98.9 96.9 4.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-65   PRO-66  5.9 6.2 -3.9 3.7 145.3 141.7 -0.3  PRO-66   GLU-67  7.5 8.2 -8.4 1.1 135.5 145.5 -3.4  GLU-67   PHE-68  9.1 9.4 -1.4 -0.7 108.7 112.0 2.0  PHE-68   LEU-69  6.6 6.3 -13.2 1.6 135.2 122.1 -2.2  LEU-69   THR-70  4.2 4.4 -16.7 1.2 154.3 153.6 -9.0  THR-70   MET-71  6.9 7.1 -0.8 6.7 112.3 132.5 -0.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees