Elongation Factor Tu

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-81   CYS-82  24.9 26.0 -21.6 20.6 22.0 23.9 -3.4  CYS-82   PRO-83  24.1 25.4 61.5 13.7 132.1 126.9 -50.4  PRO-83   GLY-84  26.8 27.8 179.4 29.7 157.4 127.7 202.6  GLY-84   HIS-85  24.3 28.5 -161.0 15.6 89.0 15.3 -224.3  HIS-85   ALA-86  21.2 29.7 -161.7 -4.3 83.8 57.3 38.4  ALA-86   ASP-87  19.8 27.3 19.3 -10.6 37.2 97.9 0.4  ASP-87   TYR-88  21.3 27.8 25.5 -56.6 39.0 42.5 -30.9  TYR-88   ILE-89  19.3 26.3 70.9 -5.1 99.0 72.1 -13.3  ILE-89   LYS-90  16.0 23.1 9.5 1.3 77.1 70.7 -0.2  LYS-90   ASN-91  17.1 21.1 0.3 8.0 13.1 9.6 10.3  ASN-91   MET-92  17.6 22.8 -3.8 -3.8 111.2 121.2 -5.7  MET-92   ILE-93  14.4 22.0 -9.2 23.0 81.2 94.0 -7.3  ILE-93   THR-94  12.9 18.3 6.0 9.9 57.8 42.1 12.0  THR-94   GLY-95  15.5 17.9 -68.4 -127.4 167.1 154.1 206.9  GLY-95   ALA-96  13.7 19.3 -34.5 -1.0 126.4 95.0 -10.8  ALA-96   ALA-97  16.9 17.7 41.2 0.6 72.8 72.2 13.5  ALA-97   GLN-98  16.7 14.2 -166.9 75.1 102.8 169.3 -82.7  GLN-98   MET-99  14.3 15.1 -78.6 -18.8 115.1 120.9 -76.7  MET-99   ASP-100  16.2 16.2 19.4 -8.3 72.7 77.0 -7.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-213   VAL-214  10.3 10.8 25.2 -55.0 53.3 58.0 -5.0  VAL-214   ARG-215  7.2 8.0 -47.9 19.9 101.2 55.6 22.8  ARG-215   ASP-216  5.3 5.2 -31.0 -33.6 63.7 78.9 71.7  ASP-216   VAL-217  3.2 2.8 136.5 -27.8 63.7 89.8 34.5  VAL-217   ASP-218  1.1 0.8 12.0 2.6 92.5 99.6 -2.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees