Alcohol Dehydrogenase E Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-175   PHE-176  4.2 4.3 0.2 -3.6 83.2 84.3 7.0  PHE-176   SER-177  1.6 1.5 1.3 3.9 19.1 21.7 33.9  SER-177   THR-178  0.7 0.6 1.2 -3.0 47.5 47.2 -16.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-290   ILE-291  11.2 11.2 5.2 -0.6 58.9 56.4 42.5  ILE-291   THR-292  11.7 11.4 -1.1 17.6 116.5 118.6 270.0  THR-292   GLY-293  9.7 9.2 4.7 -92.3 64.4 78.5 -353.6  GLY-293   VAL-294  12.7 11.2 3.7 -39.2 125.6 116.1 308.0  VAL-294   PRO-295  14.2 14.4 4.2 21.2 94.4 61.8 290.5  PRO-295   PRO-296  17.5 17.1 -23.8 2.2 101.1 42.9 166.5  PRO-296   ASP-297  20.8 17.8 -158.1 -52.3 33.6 65.6 -1528.3  ASP-297   SER-298  21.5 21.4 -105.0 -130.6 39.1 68.6 2036.7  SER-298   GLN-299  22.4 20.7 128.5 14.5 99.9 146.1 -777.0  GLN-299   ASN-300  23.1 23.2 43.4 26.5 123.0 124.0 -495.9  ASN-300   LEU-301  22.3 22.5 5.4 -10.3 69.2 66.8 -4.2  LEU-301   SER-302  22.3 22.3 -1.4 1.2 33.6 34.8 17.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-316   ALA-317  8.5 8.2 -2.4 0.2 96.4 92.3 -7.8  ALA-317   ILE-318  5.5 5.1 4.4 -3.4 40.2 42.8 -16.6  ILE-318   PHE-319  5.6 5.2 2.5 0.2 62.0 61.5 67.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-335   MET-336  9.5 9.8 6.8 -14.7 136.8 130.4 28.1  MET-336   ALA-337  12.3 12.8 10.8 2.2 95.0 91.3 -39.2  ALA-337   LYS-338  14.6 15.1 -7.3 -8.4 78.3 64.7 -31.8  LYS-338   LYS-339  12.6 13.1 2.5 13.1 42.9 52.2 53.8  LYS-339   PHE-340  12.4 12.9 -12.6 2.8 92.1 80.9 13.0  PHE-340   ALA-341  9.2 9.9 -4.5 14.5 31.6 36.5 -66.4  ALA-341   LEU-342  7.1 7.7 -0.7 -0.5 100.6 104.0 32.3  LEU-342   ASP-343  3.3 3.9 5.0 2.5 144.2 141.6 -38.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees