Restriction Endonuclease EcorII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-202   ALA-203  8.4 8.4 2.7 2.7 146.0 146.1 -22.8  ALA-203   ALA-204  6.7 6.1 -2.6 27.4 95.3 82.8 21.9  ALA-204   SER-205  8.7 8.2 -29.0 42.7 95.5 58.1 -11.6  SER-205   HIS-206  11.7 11.2 3.4 7.7 141.6 165.9 -36.6  HIS-206   TYR-207  11.9 9.2 111.3 4.4 152.2 111.8 -274.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-224   GLU-225  3.1 2.0 -2.5 -2.0 43.8 43.9 5.5  GLU-225   TYR-226  1.8 1.0 3.3 -2.2 69.4 73.6 9.2  TYR-226   ASP-227  3.0 3.4 -2.3 5.5 120.1 123.8 6.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-264   ARG-265  10.9 11.3 -9.9 -34.0 73.0 77.8 34.7  ARG-265   ALA-266  7.8 8.0 13.5 15.6 90.0 123.4 -25.7  ALA-266   GLY-267  8.8 8.3 -97.4 43.1 155.5 124.9 -127.1  GLY-267   LYS-268  8.5 9.6 29.0 48.7 75.4 56.6 103.6  LYS-268   SER-269  5.9 6.5 -67.1 9.2 47.3 55.3 138.0  SER-269   LEU-270  4.5 4.5 4.9 1.1 95.6 97.6 -2.9  LEU-270   GLU-271  7.5 7.6 5.5 9.2 36.8 37.0 24.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees