Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-85   GLY-86  6.5 6.3 -14.0 -6.8 65.0 65.0 28.8  GLY-86   PRO-87  3.4 3.4 16.1 -5.0 98.3 118.1 -24.9  PRO-87   GLU-88  5.3 5.4 -84.4 57.7 29.2 35.8 84.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-184   ASP-185  9.8 9.9 -24.4 37.5 25.2 23.5 -79.9  ASP-185   LEU-186  11.1 11.6 -26.1 0.2 36.0 37.0 105.4  LEU-186   PRO-187  9.1 9.4 -21.7 -3.0 47.1 61.6 55.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees