Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
TYR-166
ARG-167
16.6
16.5
7.3
-14.4
34.7
36.2
-3.3
ARG-167
ASP-168
14.4
14.2
14.2
0.5
38.0
31.0
-39.8
ASP-168
GLN-169
14.9
13.5
-33.4
22.7
114.3
90.9
-49.4
GLN-169
PRO-170
11.2
10.2
-35.2
-8.8
38.2
20.2
181.9
PRO-170
HIS-171
8.4
8.1
4.6
14.5
127.3
120.9
-16.5
HIS-171
PRO-172
7.2
7.6
-4.3
-1.4
114.0
110.3
-15.3
PRO-172
PHE-173
5.3
6.0
3.7
-7.8
111.0
110.9
17.5
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LYS-224
ALA-225
5.3
5.1
-3.6
10.7
114.4
123.1
-3.8
ALA-225
SER-226
8.4
8.2
-39.2
19.0
113.7
121.8
-69.1
GLN-229
SER-230
6.8
5.3
-9.6
4.5
44.0
55.7
22.7
SER-230
ASP-231
6.9
7.8
19.3
56.7
77.5
100.4
-96.3
ASP-231
ASP-232
8.1
10.1
-76.7
47.6
30.0
37.6
134.2
ASP-232
LEU-233
8.5
8.0
-21.6
-8.2
72.8
99.3
5.0
LEU-233
LEU-234
8.1
7.7
15.5
-2.6
78.6
73.3
11.6
LEU-234
THR-235
4.4
4.1
2.5
-8.8
17.9
5.6
-38.8
THR-235
HIS-236
4.5
4.3
-4.0
15.4
102.8
110.7
27.1
HIS-236
MET-237
7.8
7.6
-10.0
-11.8
62.3
83.3
6.8
MET-237
LEU-238
7.4
7.3
4.8
21.6
56.4
54.8
11.1
LEU-238
ASN-239
5.6
5.0
-5.0
27.2
162.3
158.0
110.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees