Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
PHE-165
TYR-166
16.3
16.0
10.9
-12.2
77.4
80.6
-11.1
TYR-166
ARG-167
16.6
16.5
2.4
-5.8
33.3
35.4
38.3
ARG-167
ASP-168
14.3
14.2
10.7
3.9
40.4
28.8
-53.4
ASP-168
GLN-169
14.9
13.5
-42.1
23.1
113.7
89.7
-34.8
GLN-169
PRO-170
11.1
10.0
-31.2
-8.4
36.9
18.3
161.9
PRO-170
HIS-171
8.5
8.2
11.4
11.9
125.0
117.5
-23.6
HIS-171
PRO-172
7.1
7.2
-8.7
11.9
114.7
111.7
-14.5
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LYS-224
ALA-225
5.2
5.0
-2.6
4.7
112.4
118.1
-5.5
ALA-225
SER-226
8.4
8.2
-26.2
7.1
114.6
119.8
-81.9
GLN-229
SER-230
6.8
5.6
-11.9
12.9
46.4
55.8
-24.5
SER-230
ASP-231
6.9
7.8
10.4
65.4
75.4
94.2
-63.0
ASP-231
ASP-232
8.1
10.4
-81.2
48.4
32.4
39.2
135.4
ASP-232
LEU-233
8.6
8.3
-26.2
-3.0
73.1
98.8
16.8
LEU-233
LEU-234
8.3
8.1
18.0
-7.0
80.6
74.3
-12.8
LEU-234
THR-235
4.6
4.4
0.1
-4.8
19.1
7.5
-19.2
THR-235
HIS-236
4.5
4.5
-6.3
15.9
104.2
111.5
20.8
HIS-236
MET-237
7.8
7.7
-7.7
-11.1
61.4
80.2
5.3
MET-237
LEU-238
7.5
7.3
-2.1
27.3
121.4
123.3
14.6
LEU-238
ASN-239
5.6
4.9
-8.5
30.6
163.2
159.8
99.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees