Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-103   LEU-104  6.7 6.8 3.3 -9.0 123.0 118.6 72.5
 LEU-104   PRO-105  8.6 9.0 -4.6 2.3 10.8 9.8 66.5
 PRO-105   SER-106  8.4 8.7 -1.0 -12.1 73.5 74.6 5.5
 SER-106   PHE-107  4.6 4.9 2.1 7.4 99.1 85.1 20.0
 PHE-107   SER-108  6.5 6.2 -10.9 -9.4 51.3 59.2 16.4
 SER-108   GLN-109  8.7 9.0 0.1 -2.7 131.5 133.7 -15.6
 GLN-109   GLN-110  9.2 10.2 -1.6 9.6 54.2 39.6 5.8
 GLN-110   ALA-111  6.7 8.5 -3.2 3.8 118.3 100.7 16.2
 ALA-111   MET-112  4.1 5.1 11.7 8.9 95.4 100.2 -16.4
 MET-112   LYS-113  5.6 6.1 6.2 1.9 153.9 153.7 -114.6
 LYS-113   GLY-114  5.1 5.2 -4.2 -0.4 86.5 90.9 28.9
 GLY-114   TYR-115  1.6 1.6 -9.4 1.2 127.4 123.0 -42.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLN-204   PHE-205  17.2 17.5 5.1 -7.2 122.5 117.6 29.3
 PHE-205   GLN-206  13.5 13.6 0.7 5.0 110.5 107.5 -48.9
 GLN-206   GLU-207  13.6 13.9 -2.0 -5.2 42.6 38.0 54.8
 GLU-207   ASP-208  16.7 16.8 -1.2 9.5 31.6 28.3 -46.4
 ASP-208   ILE-209  15.0 14.8 -4.2 4.7 67.5 72.2 -19.7
 ILE-209   LYS-210  12.0 11.8 -7.3 -3.1 61.0 61.4 53.8
 LYS-210   VAL-211  14.8 14.8 5.6 -11.7 154.6 155.2 15.3
 VAL-211   MET-212  16.0 15.9 6.2 -3.3 130.5 132.0 -11.5
 MET-212   ASN-213  12.5 12.4 -3.9 4.2 73.1 81.0 -1.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-232   LEU-233  4.0 4.2 -13.3 -2.4 23.2 32.9 117.6
 LEU-233   LEU-234  3.1 3.1 6.5 -5.8 116.9 117.2 -56.4
 LEU-234   THR-235  6.4 6.6 -1.1 -7.6 110.3 118.5 39.7
 THR-235   HIS-236  8.5 8.4 13.6 -10.2 118.5 103.4 -13.0
 HIS-236   MET-237  8.0 7.7 4.9 -7.0 166.2 159.0 77.2
 MET-237   LEU-238  7.8 8.2 -1.2 13.6 81.6 87.9 -124.1
 LEU-238   ASN-239  11.2 11.4 -2.5 20.7 107.7 118.5 36.6
 ASN-239   GLY-240  13.0 12.8 9.6 -2.8 90.6 100.3 10.4
 GLY-240   LYS-241  12.0 12.0 3.0 -8.4 137.0 133.8 8.6
 LYS-241   ASP-242  14.8 14.8 1.0 -0.5 98.8 103.3 15.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees