Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.4 16.1 10.5 -15.3 81.8 85.1 -39.6
 TYR-166   ARG-167  16.9 16.7 8.4 -20.4 30.4 38.2 8.2
 ARG-167   ASP-168  15.0 14.9 18.8 -3.2 39.9 33.5 4.1
 ASP-168   GLN-169  15.9 14.3 -47.5 46.3 119.6 96.1 -69.4
 GLN-169   PRO-170  12.1 10.9 -32.8 -6.3 42.9 25.6 173.7
 PRO-170   HIS-171  9.2 8.7 13.5 5.5 124.8 120.5 -43.3
 HIS-171   PRO-172  7.7 7.8 1.0 6.1 60.6 64.4 1.9
 PRO-172   PHE-173  5.8 6.3 -3.1 -9.3 73.6 73.6 20.7
 PHE-173   ILE-174  2.7 3.2 0.1 -0.6 53.4 56.7 -4.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-236   MET-237  8.4 8.2 -6.5 -14.1 57.1 76.4 34.1
 MET-237   LEU-238  8.3 8.3 11.7 13.4 59.5 56.2 -0.4
 LEU-238   ASN-239  7.0 6.4 -1.9 22.4 157.3 153.1 109.9
 ASN-239   GLY-240  7.9 7.8 -2.7 -8.4 102.7 92.7 -38.7
 GLY-240   LYS-241  11.0 11.4 0.4 -2.9 91.9 87.6 6.8
 LYS-241   ASP-242  14.4 14.5 -2.4 -1.7 79.2 71.1 4.3
 ASP-242   PRO-243  17.7 17.8 1.8 1.5 32.6 39.2 25.3
 PRO-243   GLU-244  19.7 20.2 -6.8 -0.3 83.1 83.7 -35.5
 GLU-244   THR-245  22.8 23.0 -22.3 32.6 98.4 99.0 20.3
 THR-245   GLY-246  22.2 22.0 -7.9 13.2 124.5 138.0 -3.6
 GLY-246   GLU-247  19.0 19.2 -2.6 -10.3 52.7 64.8 3.3
 GLU-247   PRO-248  18.0 17.3 -6.8 9.9 69.1 67.1 -7.4
 PRO-248   LEU-249  14.6 13.7 -4.0 -3.1 11.4 20.9 35.7
 LEU-249   ASP-250  13.9 13.1 3.0 -7.7 97.3 86.2 -9.5
 ASP-250   ASP-251  14.9 13.0 4.5 -2.5 123.6 129.3 -26.8
 ASP-251   GLU-252  12.6 10.3 2.4 -4.8 167.7 172.6 15.1
 GLU-252   ASN-253  13.7 11.8 6.5 -5.6 96.1 104.4 5.1
 ASN-253   ILE-254  13.3 12.1 6.0 -7.1 79.9 83.8 0.4
 ILE-254   ARG-255  9.8 8.7 0.3 7.0 143.5 143.8 -44.7
 ARG-255   TYR-256  8.5 7.1 -10.5 6.0 47.1 43.0 13.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees