Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-171   PRO-172  6.8 7.2 -0.3 14.7 104.1 98.0 -32.7
 PRO-172   PHE-173  6.7 7.5 -21.6 -1.2 62.7 68.5 32.8
 PHE-173   ILE-174  4.2 5.0 5.9 -3.0 55.1 55.2 19.1
 ILE-174   THR-175  1.4 2.1 5.8 9.1 34.9 31.8 36.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-230   ASP-231  8.5 8.6 -44.7 66.5 105.1 86.8 2.9
 ASP-231   ASP-232  8.4 8.5 -7.9 17.5 28.4 20.3 -24.7
 ASP-232   LEU-233  7.8 7.1 -2.2 -8.3 82.1 93.1 -15.4
 LEU-233   LEU-234  6.4 5.7 13.7 -6.9 71.0 68.9 9.7
 LEU-234   THR-235  2.6 2.0 -3.0 -1.2 171.7 175.2 -16.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-236   MET-237  5.5 5.2 -5.3 -9.7 72.2 85.4 7.0
 MET-237   LEU-238  4.9 4.8 5.2 20.0 49.6 52.1 32.1
 LEU-238   ASN-239  3.1 2.7 -10.4 24.2 167.7 160.2 67.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-258   ILE-259  3.7 3.9 0.9 5.7 3.5 6.3 43.1
 ILE-259   THR-260  4.7 5.0 1.0 -8.4 73.4 70.6 -20.4
 THR-260   PHE-261  7.4 7.8 1.1 14.6 91.4 85.9 31.6
 PHE-261   LEU-262  6.4 6.7 -6.8 1.1 128.6 141.9 -32.6
 LEU-262   ILE-263  3.7 4.1 -1.8 27.4 153.6 150.0 117.6
 ILE-263   ALA-264  5.5 6.3 3.1 1.0 88.5 94.2 35.7
 ILE-263   ALA-264  5.5 6.3 3.1 1.0 88.5 94.2 35.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-263   ALA-264  5.5 6.3 3.1 1.0 88.5 94.2 35.7
 ILE-263   ALA-264  5.5 6.3 3.1 1.0 88.5 94.2 35.7
 ALA-264   GLY-265  9.1 9.2 -8.9 2.8 103.6 76.6 -10.0
 GLY-265   HIS-266  9.0 8.3 -19.9 32.7 48.2 23.4 -131.5
 HIS-266   GLU-267  8.9 6.7 -4.2 -19.4 27.0 45.8 149.9
 GLU-267   THR-268  9.2 8.8 0.9 -33.3 101.6 93.2 95.1
 THR-268   THR-269  12.9 12.4 -6.4 -5.9 97.4 94.0 43.0
 THR-269   SER-270  14.0 13.2 -2.6 -2.4 40.0 46.0 25.1
 SER-270   GLY-271  12.6 12.8 17.8 -5.8 141.1 140.8 -65.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees