T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-553   VAL-554  23.2 22.4 41.8 -56.3 53.7 52.7 17.6
 VAL-554   GLY-555  24.7 24.0 7.0 18.2 76.3 79.8 77.6
 GLY-555   GLY-556  21.7 21.2 -3.6 -1.4 107.1 125.7 0.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-708   GLU-709  20.2 20.5 -15.2 6.7 144.1 143.3 -56.4
 GLU-709   VAL-710  20.7 20.9 3.7 -37.2 47.3 36.8 -218.7
 VAL-710   LYS-711  19.9 20.1 -26.9 -6.7 101.6 126.4 -58.5
 LYS-711   ASP-712  22.9 23.5 -161.5 -130.1 157.8 144.9 492.5
 ASP-712   LYS-713  22.8 25.1 28.7 43.9 73.8 68.4 254.0
 LYS-713   LYS-714  25.7 28.3 137.4 58.4 10.1 53.5 -1249.1
 LYS-714   THR-715  24.1 29.1 117.5 75.6 60.7 58.3 738.3
 THR-715   GLY-716  23.3 25.8 -10.5 111.0 124.6 28.3 -266.5
 GLY-716   GLU-717  19.6 25.3 -71.1 -118.7 64.1 84.7 674.5
 GLU-717   ILE-718  16.3 22.4 152.5 2.2 151.2 141.1 -1203.4
 ILE-718   LEU-719  17.9 19.8 50.4 49.2 135.1 78.7 -180.7
 LEU-719   ARG-720  18.4 16.3 162.6 18.7 134.1 146.1 1207.1
 ARG-720   LYS-721  16.8 15.5 6.9 -14.7 133.0 129.0 30.9
 LYS-721   ARG-722  18.3 17.8 20.3 16.7 163.3 154.7 -281.4
 ARG-722   SER-723  19.4 18.0 38.0 7.8 110.9 111.3 -233.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-847   GLN-848  9.6 8.2 61.2 -65.2 87.8 94.3 -57.4
 GLN-848   PHE-849  6.9 6.2 8.8 -32.7 106.2 118.4 67.7
 PHE-849   ALA-850  9.8 9.3 84.0 -54.7 163.0 135.6 -215.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-851   GLN-852  7.7 7.5 -50.7 52.7 101.7 93.7 -1.7
 GLN-852   LEU-853  9.3 7.7 -66.6 38.9 114.7 89.7 -28.1
 LEU-853   HIS-854  12.4 11.2 10.6 95.2 8.2 39.0 804.6
 HIS-854   GLU-855  13.6 14.3 -2.5 -78.3 118.3 163.8 -633.4
 GLU-855   SER-856  12.4 14.4 -17.8 44.1 68.7 114.4 -22.6
 SER-856   GLN-857  15.4 16.5 -46.9 13.5 73.2 49.9 221.4
 GLN-857   LEU-858  18.1 19.5 -34.2 147.4 10.8 53.9 -945.6
 LEU-858   ASP-859  17.5 18.0 49.8 -65.3 120.3 109.2 22.9
 ASP-859   LYS-860  16.5 21.3 114.0 -171.3 105.1 139.8 424.6
 LYS-860   MET-861  19.9 23.8 -67.8 -21.2 11.5 30.5 680.5
 MET-861   PRO-862  18.1 20.8 162.0 33.5 123.3 114.0 -1225.0
 PRO-862   ALA-863  20.2 19.4 52.7 152.4 100.2 132.5 334.6
 PRO-862   ALA-863  20.2 19.4 52.7 152.4 100.2 132.5 334.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-862   ALA-863  20.2 19.4 52.7 152.4 100.2 132.5 334.6
 PRO-862   ALA-863  20.2 19.4 52.7 152.4 100.2 132.5 334.6
 ALA-863   LEU-864  20.9 21.0 -63.1 8.4 23.8 27.9 421.1
 LEU-864   PRO-865  19.0 18.6 -4.2 -2.4 52.9 44.9 23.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees