Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-173   ILE-174  2.7 3.0 12.3 -8.9 53.3 57.4 14.3
 ILE-174   THR-175  1.4 1.1 10.5 1.2 32.3 30.1 29.5
 THR-175   SER-176  1.7 1.9 -9.3 6.2 81.5 79.5 8.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-223   LYS-224  4.8 5.0 26.3 -5.5 29.8 36.3 99.1
 LYS-224   ALA-225  5.4 4.7 9.8 -40.7 67.0 51.8 -84.2
 ALA-225   SER-226  8.9 8.0 16.2 -5.3 73.4 85.6 4.3
 GLN-229   SER-230  8.7 6.3 -2.4 5.0 39.3 54.1 17.9
 SER-230   ASP-231  8.3 7.8 21.5 11.8 77.1 80.1 11.0
 ASP-231   ASP-232  9.1 9.1 -77.9 74.9 27.1 24.8 20.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees