Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  15.9 15.4 7.7 -7.6 77.8 78.9 -0.9
 TYR-166   ARG-167  16.2 15.8 8.1 -17.8 34.6 36.4 12.6
 ARG-167   ASP-168  13.9 13.4 13.9 -5.7 39.3 36.0 -97.3
 ASP-168   GLN-169  14.4 12.2 -33.8 26.6 112.9 90.1 -13.8
 GLN-169   PRO-170  10.6 9.0 -43.7 -9.1 36.5 15.5 226.1
 PRO-170   HIS-171  8.0 7.4 5.3 18.6 126.2 120.9 -59.3
 HIS-171   PRO-172  6.7 6.7 -3.2 3.9 113.5 108.8 -11.7
 PRO-172   PHE-173  4.6 5.0 -3.7 -5.5 67.3 67.2 11.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.4 5.0 -1.3 9.6 112.5 121.8 -4.3
 ALA-225   SER-226  8.5 8.2 -33.1 13.4 113.4 120.2 -74.0
 GLN-229   SER-230  6.9 5.4 -1.9 -3.8 45.3 53.9 -1.3
 SER-230   ASP-231  6.8 7.8 18.8 53.7 75.6 100.4 -67.4
 ASP-231   ASP-232  8.0 10.1 -80.7 57.5 31.5 37.6 108.5
 ASP-232   LEU-233  8.4 8.0 -19.2 -13.7 74.0 103.0 8.0
 LEU-233   LEU-234  8.1 7.8 19.1 -3.3 79.3 73.3 16.2
 LEU-234   THR-235  4.4 4.2 1.0 -9.8 17.8 5.7 -34.3
 THR-235   HIS-236  4.4 4.4 0.0 13.1 75.4 68.0 30.1
 HIS-236   MET-237  7.7 7.7 -11.8 -6.3 62.7 83.0 0.2
 MET-237   LEU-238  7.4 7.3 3.2 21.8 57.3 53.3 15.8
 LEU-238   ASN-239  5.6 4.9 -1.4 17.8 163.9 157.7 88.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees