Elongation Factor Tu

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-255   VAL-256  11.7 12.7 -1.5 2.0 40.0 36.2 25.8  VAL-256   GLY-257  11.9 12.9 4.6 -7.8 113.6 119.7 -1.1  GLY-257   LEU-258  10.1 11.1 5.9 -0.8 120.9 115.9 -20.3  LEU-258   ALA-259  7.4 8.3 -40.8 41.1 51.9 58.6 23.1  ALA-259   PRO-260  9.1 10.0 7.4 7.1 118.7 124.6 -44.4  PRO-260   GLU-261  11.9 12.8 -0.8 -19.9 123.7 117.8 -107.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-309   ILE-310  20.5 20.8 -8.9 -17.5 56.0 48.2 140.3  ILE-310   THR-311  18.4 18.7 1.2 4.5 166.6 146.8 -58.9  THR-311   PRO-312  18.6 18.4 44.1 -17.7 111.3 125.0 -117.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-387   ILE-388  17.5 17.5 -1.2 5.4 161.2 159.9 22.7  ILE-388   ARG-389  17.5 17.5 2.6 -6.6 84.0 85.6 22.7  ARG-389   GLU-390  16.8 16.8 -0.1 41.4 160.4 155.8 264.3  GLU-390   GLY-391  17.1 16.8 -32.8 11.2 97.9 76.8 -13.4  GLY-391   GLY-392  18.1 15.5 -175.5 -170.6 34.6 13.3 -112.4  GLY-392   ARG-393  18.5 17.2 22.3 5.3 125.9 103.6 -71.6  ARG-393   THR-394  20.4 19.9 -32.2 9.7 78.9 82.1 -31.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees