T7 DNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-530   GLY-531  7.3 7.2 31.0 -65.7 145.5 129.2 101.0  GLY-531   ALA-532  5.3 5.0 28.9 -14.8 93.2 91.2 5.9  ALA-532   GLY-533  1.8 2.1 2.2 15.0 133.0 116.8 -29.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees