Alcohol Dehydrogenase (Holo Form) (E.C.1.1.1.1)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-175   PHE-176  2.9 3.1 -6.3 5.5 88.5 90.0 -22.0  PHE-176   SER-177  0.9 0.7 0.5 1.3 30.5 29.4 55.4  SER-177   THR-178  3.1 2.9 -1.6 0.5 139.4 137.5 -21.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-288   SER-289  14.0 14.1 1.5 2.5 50.8 47.3 32.3  SER-289   VAL-290  12.5 12.5 1.8 -9.3 93.2 91.3 14.0  VAL-290   ILE-291  9.0 8.9 0.0 -15.2 67.3 61.3 -90.9  ILE-291   VAL-292  8.6 8.2 27.6 -23.0 60.3 71.7 129.7  VAL-292   GLY-293  6.7 5.8 24.9 -62.7 76.8 75.9 -131.4  GLY-293   VAL-294  9.7 8.6 -18.1 -63.0 47.9 41.4 773.1  VAL-294   PRO-295  10.9 10.9 -4.0 15.0 76.5 100.7 73.4  PRO-295   PRO-296  14.0 13.7 -24.9 8.3 90.6 32.4 40.6  PRO-296   ASP-297  17.4 14.5 -160.8 -50.9 27.8 62.1 -1556.3  ASP-297   SER-298  17.8 17.8 -110.8 -115.5 39.4 71.1 1833.3  SER-298   GLN-299  18.3 16.6 114.9 24.1 95.1 148.3 -834.8  GLN-299   ASN-300  19.1 19.0 35.3 25.0 114.4 115.5 -262.6  ASN-300   LEU-301  18.1 17.8 -2.5 10.9 110.7 114.2 24.2  LEU-301   SER-302  18.2 18.0 3.3 -4.1 147.0 143.6 -33.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-317   ILE-318  1.8 1.6 7.3 -7.7 46.4 48.9 -3.9  ILE-318   PHE-319  3.0 2.9 8.7 -7.7 52.7 54.0 103.3  PHE-319   GLY-320  2.7 3.0 4.1 0.0 92.0 92.4 -36.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees