Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-87   GLU-88  6.6 5.2 -25.3 55.1 125.7 123.1 126.7  GLU-88   ILE-89  4.0 3.7 3.4 -20.0 118.3 117.7 7.1  ILE-89   ARG-90  2.1 2.6 -6.3 6.2 106.5 108.3 -7.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  4.0 4.4 0.7 -2.0 102.4 105.8 -0.8  ASN-178   LEU-179  2.0 2.5 -4.6 6.4 89.7 90.0 -0.5  LEU-179   PRO-180  5.2 5.3 -0.8 -8.0 63.4 58.6 82.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  CYS-182   ASP-183  9.7 10.0 164.4 -150.1 136.6 109.9 -58.2  ASP-183   VAL-184  10.1 10.6 19.5 8.9 143.9 145.0 -156.8  VAL-184   ASP-185  7.1 7.6 -28.6 16.1 49.8 51.5 90.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-187   ALA-188  9.6 9.1 14.6 -14.5 136.3 130.4 -9.5  ALA-188   VAL-189  12.0 10.6 19.5 0.6 77.8 58.0 166.3  VAL-189   SER-190  12.4 11.5 -24.8 11.6 153.2 150.5 -83.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees