Heme Oxygenase-1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-46   PHE-47  7.0 7.0 2.3 -1.9 31.1 36.3 -46.3  PHE-47   LYS-48  4.7 4.7 30.4 -27.8 44.1 38.6 15.5  LYS-48   LEU-49  4.0 4.5 17.5 -16.1 63.1 62.7 18.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-137   LEU-138  14.0 14.6 11.0 8.4 57.9 65.4 33.9  LEU-138   GLY-139  17.5 18.1 5.0 -4.0 20.1 4.7 8.9  GLY-139   ASP-140  17.2 17.1 -16.7 35.4 121.9 101.8 34.2  ASP-140   LEU-141  13.8 13.9 -4.6 -4.2 92.7 88.0 6.9  LEU-141   SER-142  15.1 16.4 55.7 -125.9 49.2 45.1 -282.6  SER-142   GLY-143  18.6 18.7 -24.6 34.1 133.9 155.8 57.8  GLY-143   GLY-144  17.2 17.8 -114.0 -4.6 73.7 97.4 82.0  GLY-144   GLN-145  16.1 14.1 85.0 5.9 48.5 67.5 340.6  GLN-145   VAL-146  15.6 15.2 -80.6 50.8 160.0 175.7 -174.6  VAL-146   LEU-147  15.6 16.0 -29.9 59.1 114.4 110.7 15.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-159   SER-160  13.8 14.3 -3.3 14.6 165.9 158.9 57.8  SER-160   GLY-161  14.2 14.2 -11.0 -8.3 117.8 124.7 -30.6  GLY-161   GLU-162  10.9 10.8 17.6 -21.7 84.3 87.5 55.4  GLU-162   GLY-163  8.8 8.6 4.3 -12.0 123.1 118.4 11.4  GLY-163   LEU-164  5.1 4.9 31.1 -24.8 82.7 79.9 -0.3  LEU-164   ALA-165  6.1 6.3 -29.4 30.8 154.1 158.5 -0.9  ALA-165   PHE-166  6.4 6.7 -16.9 19.2 114.6 111.5 -22.1  PHE-166   PHE-167  6.1 6.2 -23.3 15.2 79.0 82.9 -12.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees