DynDom - Protein Domain Motions

Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	Crispr-Associated Endonuclease Cas9/csn1
Conformer 1 (PDB)	6o0y (A)
Conformer 2 (PDB)	6o0z (A)
Window Length	5
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	882	3.79	5 - 669 829 - 839 841 - 1284
2	132	2.47	670 - 828 840 - 840

Sequence

`________________________________________________________________________________________________________________________` `6o0y(A) : KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `:` `6o0z(A) : KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDL--------------------------------------------------------------------` `:` `6o0z(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDdVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIAL` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) :` `-------------------------------------------------------------eITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ` `:` `6o0z(A) :` `SLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIdLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQ` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQ` `:` `6o0z(A) : EEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNReKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQ` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : SFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDN` `:` `6o0z(A) : SFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKY--------aFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE---------------aSLGTYHDLLKIIKDKDFLDN` `________________________________________________________________________________________________________________________` `_________________________________________________________669____________________________________________________________` `6o0y(A) : EENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQdSLHEHIANLAGSPAIK` `:` `6o0z(A) : EENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIR-----kTILDFLKS----nRNFMQLIHDDSLTFKEDIQKAQdSLHEHIANLAGSPAIK` `_________________________________________________________669____________________________________________________________` `___________________________________________________________________________________________828__________________________` `6o0y(A) : KGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN` `:` `6o0z(A) : KGILQTVKVVDELVKVMGRHKPENIVIEMAR--------------rMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQ--lKDDSIDN` `___________________________________________________________________________________________828__________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSE---aGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK` `:` `6o0z(A) : KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : VREINNYHHAHDAYLNAVVGTALIKKY----------------------------------------------------------kGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESI` `:` `6o0z(A) : VREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYkVYDVRKMIyFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : LPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPS` `:` `6o0z(A) : LPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPS` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `6o0y(A) : KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ` `:` `6o0z(A) : KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ` `________________________________________________________________________________________________________________________` `________________` `6o0y(A) : SITGLYETRIDLSQLG` `:` `6o0z(A) : SITGLYETRIDLSQLG` `________________`

Morph

Morph animation in progress.
Status: Initialising

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Property	Value
Fixed Domain ( blue )	1
Moving Domain ( red )	2
Rotation Angle (deg)	140.7
Translation (A)	-1.3
Closure (%)	3.2
Bending Residues ( green )	669 - 670 828 - 841
Bending Region Analysis

Dynamic Contact Graph

Conformer 1 Contact:
Residue 1 ———› Residue 2

Conformer 2 Contact:
Residue 2 ———› Residue 1

Movement classification: Hinge

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