DynDom - Protein Domain Motions

Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	Crispr-Associated Endonuclease Cas9/csn1
Conformer 1 (PDB)	5f9r (B)
Conformer 2 (PDB)	7s4u (A)
Window Length	7
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	771	2.16	6 - 178 307 - 497 721 - 1361
2	177	1.55	498 - 702

Sequence

`________________________________________________________________________________________________________________________` `5f9r(B) : KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `:` `7s4u(A) : KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `:` `7s4u(A) : VDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGY` `:` `7s4u(A) : ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI-----dLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGY` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : IDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVV` `:` `7s4u(A) : IDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVV` `________________________________________________________________________________________________________________________` `____________497_________________________________________________________________________________________________________` `5f9r(B) : DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK` `:` `7s4u(A) : DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKY--------aFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE---------------aSLGTYHDLLKIIK` `____________497_________________________________________________________________________________________________________` `______________________________________________________________________________________________699_______________________` `5f9r(B) : DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHE` `:` `7s4u(A) : DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIR-----kTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQ-----dSLHE` `______________________________________________________________________________________________699_______________________` `________________________________________________________________________________________________________________________` `5f9r(B) : HIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV` `:` `7s4u(A) : HIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEM-------------------------------------------------------------------------------` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) :` `PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL` `:` `7s4u(A) :` `---------------------------------------------------------------------------------tRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFAT` `:` `7s4u(A) : VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK--------------------------------------------------------------------------dKGRDFAT` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : VRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS` `:` `7s4u(A) : VRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `5f9r(B) : LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPA` `:` `7s4u(A) : LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPA` `________________________________________________________________________________________________________________________` `____________________________________________` `5f9r(B) : AFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ**` `:` `7s4u(A) : AFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG` `____________________________________________`

Morph

Morph animation in progress.
Status: Initialising

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Property	Value
Fixed Domain ( blue )	1
Moving Domain ( red )	2
Rotation Angle (deg)	21.0
Translation (A)	-0.9
Closure (%)	96.0
Bending Residues ( green )	497 - 498 699 - 721
Bending Region Analysis

Dynamic Contact Graph

Conformer 1 Contact:
Residue 1 ———› Residue 2

Conformer 2 Contact:
Residue 2 ———› Residue 1

Movement classification: Hinge

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