TYROSYL-TRNA SYNTHETASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1h3e(A) : *HTPEEALALLKRGAEEIVPEEELLAKLKEGRPLTVKLGADPTRPDLHLG 
         :                                                    
 1h3f(B) : GHTPEEALALLKRGAEEIVPEEELLAKLKEGRPLTVKLGADPTRPDLHLG 
           __________________________________________________ 

           __________________________________________________ 
 1h3e(A) : HAVVLRKMRQFQELGHKVVLIIGDFTGMIGDPSGRSKTRPPLTLEETREN 
         :                                                    
 1h3f(B) : HAVVLRKMRQFQELGHKVVLIIGDFTGMI-------------tLEETREN 
           __________________________________________________ 

           __________________________________________________ 
 1h3e(A) : AKTYVAQAGKILRQEPHLFELRYNSEWLEGLTFKEVVRLTSLMTVAQMLE 
         :                                                    
 1h3f(B) : AKTYVAQAGKILRQEPHLFELRYNSEWLEGLTFKEVVRLTSLMTVAQMLE 
           __________________________________________________ 

           __________________________________________________ 
 1h3e(A) : REDFKKRYEAGIPISLHELLYPFAQAYDSVAIRADVEMGGTDQRFNLLVG 
         :                                                    
 1h3f(B) : REDFKKRYEAGIPISLHELLYPFAQAYDSVAIRADVEMGGTDQRFNLLVG 
           __________________________________________________ 

           __________________________________________________ 
 1h3e(A) : REVQRAYGQSPQVCFLMPLLVGLDGREKMSKSLDNYIGLTEPPEAMFKKL 
         :                                                    
 1h3f(B) : REVQRAYGQSPQVCFLMPLLVGLDGREKMSKSLDNYIGLTEPPEAMFKKL 
           __________________________________________________ 

           __________________________________________________ 
 1h3e(A) : MRVPDPLLPSYFRLLTDLEEEEIEALLKAGPVPAHRVLARLLTAAYALPQ 
         :                                                    
 1h3f(B) : MRVPDPLLPSYFRLLTDLEEEEIEALLKAGPVPAHRVLARLLTAAYALPQ 
           __________________________________________________ 

           ___________________________________342____________ 
 1h3e(A) : IPPRIDRAFYESLGYAWEAFGRDKEAGPEEVRRAEARYDEVAKGGIPEEI 
         :                                                    
 1h3f(B) : IPPRIDRAFYESLGYAWEAFGRDKEAGPEEVRRAEARYDe---------I 
           ___________________________________342____________ 

           __________________________________________________ 
 1h3e(A) : PEVTIPASELKEGRIWVARLFTLAGLTPSNAEARRLIQNRGLRLDGEVLT 
         :                                                    
 1h3f(B) : PEVTIPASELKEGRIWVARLFTLAGLTPSNAEARRLIQNRGLRLDGEVLT 
           __________________________________________________ 

           ____________________________                       
 1h3e(A) : DPMLQVDLSRPRILQRGKDRFVRVRLSD                       
         :                                                    
 1h3f(B) : DPMLQVDLSRPRILQRGKDRFVRVRLSD                       
           ____________________________                       

Details

  Property   Value
 Conformer 1 1h3e(A)
 Conformer 2 1h3f(B)
 Radius gyration for conformer 1 25.33 A
 Radius gyration for conformer 2 29.97 A
 EC Number 6.1.1.1  
Click here to see the DynDom results or the famliy
 Trigger ligands ATP1433
TYB1434
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands No
 Residues in enzyme contacting ligands ALA44  ARG93  ASN128  ASP182  ASP196  ASP45  ASP85  GLN179  GLN197  GLU191  GLY193  GLY194  GLY43  GLY54  HIS52  HIS55  ILE75  LEU223  LEU224  LYS232  LYS235  LYS41  MET233  PRO222  SER234  TYR108  TYR175  VAL57  VAL58 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT