GUANYLATE KINASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________229_____________________ 
 1ex6(B) : SRPIVISGPSGTGKSTLLKKLFAEYPDSFGFSVSSTTRTPRAGEVNGKDY 
         :                                                    
 1ex7(A) : SRPIVISGPSGTGKSTLLKKLFAEYPDSFGFSVSSTTRTPRAGEVNGKDY 
           ___________________________29_____________________ 

           ________________________________285_______________ 
 1ex6(B) : NFVSVDEFKSMIKNNEFIEWAQFSGNYYGSTVASVKQVSKSGKTCILDID 
         :                                                    
 1ex7(A) : NFVSVDEFKSMIKNNEFIEWAQFSGNYYGSTVASVKQVSKSGKTCILDID 
           _________________________________85_______________ 

           __________________________________________________ 
 1ex6(B) : MQGVKSVKAIPELNARFLFIAPPSVEDLKKRLEGRGTETEESINKRLSAA 
         :                                                    
 1ex7(A) : MQGVKSVKAIPELNARFLFIAPPSVEDLKKRLEGRGTETEESINKRLSAA 
           __________________________________________________ 

           ____________________________________               
 1ex6(B) : QAELAYAETGAHDKVIVNDDLDKAYKELKDFIFAEK               
         :                                                    
 1ex7(A) : QAELAYAETGAHDKVIVNDDLDKAYKELKDFIFAEK               
           ____________________________________               

Details

  Property   Value
 Conformer 1 1ex6(B)
 Conformer 2 1ex7(A)
 Radius gyration for conformer 1 17.69 A
 Radius gyration for conformer 2 16.51 A
 EC Number 2.7.4.8  
Click here to see the DynDom results or the famliy
 Trigger ligands 5GP187
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG38  ARG41  ASP100  ASP98  GLU69  GLY103  GLY79  ILE99  PHE73  SER34  SER80  TYR50  TYR78 
 Residues in extended bending regions contacting ligands SER34 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands SER34 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT