Nickel-binding periplasmic protein
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2noo(A) : DEITTAWPVNVGPLNPHLATPNQMFAQSMVYEPLVKYQADGSVIPWLAKS 
         :                                                    
 1uiu(A) : DEITTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKYQADGSVIPWLAKS 
           __________________________________________________ 

           __________________________________________________ 
 2noo(A) : WTHSEDGKTWTFTLRDDVKFSNGEPFDAEAAAENFRAVLDNRQAHAALEL 
         :                                                    
 1uiu(A) : WTHSEDGKTWTFTLRDDVKFSNGEPFDAEAAAENFRAVLDNRQRHAWLEL 
           __________________________________________________ 

           __________________________________________________ 
 2noo(A) : ANQIVDVKALSKTELQITLKSAYYPFLQELALPAPFRFIAPSQFKNHETM 
         :                                                    
 1uiu(A) : ANQIVDVKALSKTELQITLKSAYYPFLQELALPRPFRFIAPSQFKNHETM 
           __________________________________________________ 

           __________________________________________________ 
 2noo(A) : NGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITFNVIPDPT 
         :                                                    
 1uiu(A) : NGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITFNVIPDPT 
           __________________________________________________ 

           ____________________226__________________247______ 
 2noo(A) : TRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLAL 
         :                                                    
 1uiu(A) : TRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLAL 
           ____________________226__________________247______ 

           __________________________________________________ 
 2noo(A) : NTAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVP-aNL 
         :                                                    
 1uiu(A) : NTAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANL 
           __________________________________________________ 

           __________________________________________________ 
 2noo(A) : GLKPSQYDPQKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALS 
         :                                                    
 1uiu(A) : GLKPSQYDPQKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALS 
           __________________________________________________ 

           _________________________________________397______ 
 2noo(A) : KSMAEIIQADMRQIGADVSLIGEEESSI-aRQRDGRFGMIFHRTAGAPAD 
         :                                                    
 1uiu(A) : KSMAEIIRADMRQIGADVSLIGEEESSIYARQRDGRFGMIFHRTWGAPYD 
           _________________________________________397______ 

           __________________________________________________ 
 2noo(A) : PHAFLSSMRVPSHADFQAQQGLADKPLIDKEIGEVLATHDETQRQALYRD 
         :                                                    
 1uiu(A) : PHAFLSSMRVPSHADFQAQQGLADKPLIDKEIGEVLATHDETQRQALYRD 
           __________________________________________________ 

           _____________469___475_______485____492_______     
 2noo(A) : ILTRLHDEAVYLPISYISMMVVSKPELGNIPYAPIATEIPFEQIKP     
         :                                                    
 1uiu(A) : ILTRLHDEAVYLPISYISMMVVSKPELGNIPYAPIATEIPFEQIKP     
           _____________469___475_______485____492_______     

Details

  Property   Value
 Conformer 1 2noo(A)
 Conformer 2 1uiu(A)
 Radius gyration for conformer 1 24.34 A
 Radius gyration for conformer 2 24.33 A
 EC Number 3.6.3.24  
Click here to see the DynDom results or the famliy
 Trigger ligands NI1001
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact No
 Spanning ligands Yes
 Residues in enzyme contacting ligands HIS442  HIS56 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT