Glyoxylate reductase/hydroxypyruvate reductas
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2h1s(C) : *LkVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVAGAHGL 
         :                                                    
 2gcg(B) : LMKVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVAGAHGL 
           __________________________________________________ 

           ____________________________________________102___ 
 2h1s(C) : LCLLSDHVDKRILDAAGANLKVIST-sVGIDHLALDEIKKRGIRVGYTPD 
         :                                                    
 2gcg(B) : LCLLSDHVDKRILDAAGANLKVISTMSVGIDHLALDEIKKRGIRVGYTPD 
           ____________________________________________102___ 

           __________________________________________________ 
 2h1s(C) : VLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQST 
         :                                                    
 2gcg(B) : VLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYGLTQST 
           __________________________________________________ 

           __________________________________________________ 
 2h1s(C) : VGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPEL 
         :                                                    
 2gcg(B) : VGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPEL 
           __________________________________________________ 

           __________________________________________________ 
 2h1s(C) : AAQSDFIVVACSLTPATEGLCNKDFFQK-kETAVFINISRGDVVNQDDLY 
         :                                                    
 2gcg(B) : AAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVNQDDLY 
           __________________________________________________ 

           ______________________________________296_________ 
 2h1s(C) : QALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNT- 
         :                                                    
 2gcg(B) : QALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNTM 
           ______________________________________296_________ 

           _______________________                            
 2h1s(C) : sLLAANNLLAGLRGEP-pSEL**                            
         :                                                    
 2gcg(B) : SLLAANNLLAGLRGEPMPSELKL                            
           _______________________                            

Details

  Property   Value
 Conformer 1 2h1s(C)
 Conformer 2 2gcg(B)
 Radius gyration for conformer 1 21.96 A
 Radius gyration for conformer 2 21.72 A
 EC Number 1.1.1.79  
Click here to see the DynDom results or the famliy
 Trigger ligands NDP2002
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA215  ARG163  ARG185  ARG188  ARG245  ASP269  CYS216  GLN186  GLY160  GLY162  GLY184  GLY295  GLY84  HIS293  ILE159  ILE164  ILE243  LEU161  SER217  SER244  SER296  THR111  THR183  THR222  VAL83 
 Residues in extended bending regions contacting ligands GLY295  HIS293  SER296 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands GLY295  SER296 
  Residues in extended bending regions with an H-bond between its main chain and ligands GLY295  SER296 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT