PHENOL HYDROXYLASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1pn0(A) : TKYSESYCDVLIVGAGPAGLMAARVLSEYVRQKPDLKVRIIDKRSTKVYN 
         :                                                    
 1pn0(D) : TKYSESYCDVLIVGAGPAGLMAARVLSEYVRQKPDLKVRIIDKRSTKVYN 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : GQADGLQCRTLESLKNLGLADKILSEANDMSTIALYNPDENGHIRRTDRI 
         :                                                    
 1pn0(D) : GQADGLQCRTLESLKNLGLADKILSEANDMSTIALYNPDENGHIRRTDRI 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : PDTLPGISRYHQVVLHQGRIERRILDSIAEISDTRIKVERPLIPEKMEID 
         :                                                    
 1pn0(D) : PDTLPGISRYHQVVLHQGRIERRILDSIAEISDTRIKVERPLIPEKMEID 
           __________________________________________________ 

           ___________________172____________________________ 
 1pn0(A) : SSKAEDPEAYPVTMTLRYMSEDESTPLQFGHKTENGLFRSNLQTQEEEDA 
         :                                                    
 1pn0(D) : SSKAEDPEAYPVTMTLRYMSEDESTPLQFGHKTENGLFRSNLQTQEEEDA 
           ___________________172____________________________ 

           _204______________________________________________ 
 1pn0(A) : NYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGV 
         :                                                    
 1pn0(D) : NYRLPEGKEAGEIETVHCKYVIGCDGGHSWVRRTLGFEMIGEQTDYIWGV 
           _204______________________________________________ 

           __________________________________________________ 
 1pn0(A) : LDAVPASNFPDIRSRCAIHSAESGSIMIIPRENNLVRFYVQLQA----tK 
         :                                                    
 1pn0(D) : LDAVPASNFPDIRSRCAIHSAESGSIMIIPRENNLVRFYVQLQArVDRTK 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : FTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAG 
         :                                                    
 1pn0(D) : FTPEVVIANAKKIFHPYTFDVQQLDWFTAYHIGQRVTEKFSKDERVFIAG 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : DACHTHSPKAGQGMNTSMMDTYNLGWKLGLVLTGRAKRDILKTYEEERQP 
         :                                                    
 1pn0(D) : DACHTHSPKAGQGMNTSMMDTYNLGWKLGLVLTGRAKRDILKTYEEERQP 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : FAQALIDFDHQFSRLFSGRPAKDVADEMGVSMDVFKEAFVKGNEFASGTA 
         :                                                    
 1pn0(D) : FAQALIDFDHQFSRLFSGRPAKDVADEMGVSMDVFKEAFVKGNEFASGTA 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : INYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSEGLWMHFGDRLVT 
         :                                                    
 1pn0(D) : INYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSEGLWMHFGDRLVT 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : DGRFRIIVFAGKATDATQMSRIKKFAAYLDSENSVISRYTPKGADRNSRI 
         :                                                    
 1pn0(D) : DGRFRIIVFAGKATDATQMSRIKKFAAYLDSENSVISRYTPKGADRNSRI 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : DVITIHSCHRDDIEMHDFPAPALHPKWQYDFIYADCDSWHHPHPKSYQAW 
         :                                                    
 1pn0(D) : DVITIHSCHRDDIEMHDFPAPALHPKWQYDFIYADCDSWHHPHPKSYQAW 
           __________________________________________________ 

           __________________________________________________ 
 1pn0(A) : GVDETKGAVVVVRPDGYTSLVTDLEGTAEIDRYFSGILVEPKEKSGAQTE 
         :                                                    
 1pn0(D) : GVDETKGAVVVVRPDGYTSLVTDLEGTAEIDRYFSGILVEPKEKSGAQTE 
           __________________________________________________ 

           ______                                             
 1pn0(A) : ADWTKS                                             
         :                                                    
 1pn0(D) : ADWTKS                                             
           ______                                             

Details

  Property   Value
 Conformer 1 1pn0(A)
 Conformer 2 1pn0(D)
 Radius gyration for conformer 1 26.46 A
 Radius gyration for conformer 2 26.62 A
 EC Number 1.14.13.7  
Click here to see the DynDom results or the famliy
 Trigger ligands FAD6041
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA15  ALA18  ALA53  ARG189  ARG287  ARG44  ASN371  ASN50  ASP225  ASP357  ASP42  ASP54  CYS224  GLN368  GLN52  GLY14  GLY16  GLY226  GLY356  GLY367  GLY369  GLY55  ILE143  LEU142  LYS43  MET370  PRO144  PRO17  PRO364  SER373  THR334  TRP230  TYR289  VAL13  VAL250 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT