3-PHOSPHOGLYCERATE KINASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 16pk    : EKKSINECDLKGKKVLIRVDFNVPVKNGKITNDYRIRSALPTLKKVLTEG 
         :                                                    
 13pk(C) : EKKSINECDLKGKKVLIRVDFNVPVKNGKITNDYRIRSALPTLKKVLTEG 
           __________________________________________________ 

           __________________________________________________ 
 16pk    : GSCVLMSHLGRPKGIPMAQAGKIRSTGGVPGFQQKATLKPVAKRLSELLL 
         :                                                    
 13pk(C) : GSCVLMSHLGRPKGIPMAQAGKIRSTGGVPGFQQKATLKPVAKRLSELLL 
           __________________________________________________ 

           __________________________________________________ 
 16pk    : RPVTFAPDCLNAADVVSKMSPGDVVLLENVRFYKEEGSKKAKDREAMAKI 
         :                                                    
 13pk(C) : RPVTFAPDCLNAADVVSKMSPGDVVLLENVRFYKEEGSKKAKDREAMAKI 
           __________________________________________________ 

           ____________________________________________201___ 
 16pk    : LASYGDVYISDAFGTAHRDSATMTGIPKILGNGAAGYLMEKEISYFAKVL 
         :                                                    
 13pk(C) : LASYGDVYISDAFGTAHRDSATMTGIPKILGNGAAGYLMEKEISYFAKVL 
           ____________________________________________201___ 

           _________216______________________________________ 
 16pk    : GNPPRPLVAIVGGAKVSDKIQLLDNMLQRIDYLLIGGAMAYTFLKAQGYS 
         :                                                    
 13pk(C) : GNPPRPLVAIVGGAKVSDKIQLLDNMLQRIDYLLIGGAMAYTFLKAQGYS 
           _________216______________________________________ 

           __________________________________________________ 
 16pk    : IGKSKCEESKLEFARSLLKKAEDRKVQVILPIDHVCHTEFKAVDSPLITE 
         :                                                    
 13pk(C) : IGKSKCEESKLEFARSLLKKAEDRKVQVILPIDHVCHTEFKAVDSPLITE 
           __________________________________________________ 

           __________________________________________________ 
 16pk    : DQNIPEGHMALDIGPKTIEKYVQTIGKCKSAIWNGPMGVFEMVPYSKGTF 
         :                                                    
 13pk(C) : DQNIPEGHMALDIGPKTIEKYVQTIGKCKSAIWNGPMGVFEMVPYSKGTF 
           __________________________________________________ 

           __________________________________391_____________ 
 16pk    : AIAKAMGRGTHEHGLMSIIGGGDSASAAELSGEAKRMSHVSTGGGASLEL 
         :                                                    
 13pk(C) : AIAKAMGRGTHEHGLMSIIGGGDSASAAELSGEAKRMSHVSTGGGASLEL 
           __________________________________391_____________ 

           _______________                                    
 16pk    : LEGKTLPGVTVLDDK                                    
         :                                                    
 13pk(C) : LEGKTLPGVTVLDDK                                    
           _______________                                    

Details

  Property   Value
 Conformer 1 16pk( )
 Conformer 2 13pk(C)
 Radius gyration for conformer 1 23.15 A
 Radius gyration for conformer 2 21.9 A
 EC Number 2.7.2.3  
Click here to see the DynDom results or the famliy
 Trigger ligands ADP421
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA218  ALA242  ALA314  ASN338  ASP377  GLU345  GLY217  GLY241  GLY339  GLY342  GLY375  GLY376  LEU315  LYS219  LYS223  PHE294  PRO340  SER378  TYR245  VAL343 
 Residues in extended bending regions contacting ligands ALA218  GLY217  LYS219  LYS223 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA218  LYS223 
  Residues in extended bending regions with an H-bond between its main chain and ligands ALA218 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT