R-alcohol dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1zk3(C) : SNRLDGKVAIITGGTLGIGLAIATKFVEEGAKVMITDRHSDVGEKAAKSV 
         :                                                    
 1zk4(A) : SNRLDGKVAIITGGTLGIGLAIATKFVEEGAKVMITGRHSDVGEKAAKSV 
           __________________________________________________ 

           __________________________________________________ 
 1zk3(C) : GTPDQIQFFQHDSSDEDGWTKLFDATEKAFGPVSTLVNNAGIAVNKSVEE 
         :                                                    
 1zk4(A) : GTPDQIQFFQHDSSDEDGWTKLFDATEKAFGPVSTLVNNAGIAVNKSVEE 
           __________________________________________________ 

           __________________________________________________ 
 1zk3(C) : TTTAEWRKLLAVNLDGVFFGTRLGIQRMKNKGLGASIINMSSIEGFVGDP 
         :                                                    
 1zk4(A) : TTTAEWRKLLAVNLDGVFFGTRLGIQRMKNKGLGASIINMSSIEGFVGDP 
           __________________________________________________ 

           ________________________________185_______________ 
 1zk3(C) : SLGAYNASKGAVRIMSKSAALDCALKDYDVRVNTVHPGYIKTPLVDDLPG 
         :                                                    
 1zk4(A) : SLGAYNASKGAVRIMSKSAALDCALKDYDVRVNTVHPGYIKTPLVDDLPG 
           ________________________________185_______________ 

           ____207___________________________________________ 
 1zk3(C) : AEEAMSQRTKTPMGHIGEPNDIAYICVYLASNESKFATGSEFVVDGGYTA 
         :                                                    
 1zk4(A) : AEEAMSQRTKTPMGHIGEPNDIAYICVYLASNESKFATGSEFVVDGGYTA 
           ____207___________________________________________ 

           _                                                  
 1zk3(C) : Q                                                  
         :                                                    
 1zk4(A) : Q                                                  
           _                                                  

Details

  Property   Value
 Conformer 1 1zk3(C)
 Conformer 2 1zk4(A)
 Radius gyration for conformer 1 17.34 A
 Radius gyration for conformer 2 17.48 A
 EC Number 1.1.1.2  
Click here to see the DynDom results or the famliy
 Trigger ligands NAP1270
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA90  ARG38  ASN89  ASP62  GLY13  GLY14  GLY17  GLY188  GLY19  GLY37  GLY91  HIS61  ILE18  ILE190  ILE92  LEU16  LEU194  MET140  SER63  THR15  THR192  THR36  TYR155  VAL112  VAL195 
 Residues in extended bending regions contacting ligands GLY188  ILE190  THR192 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ILE190 
  Residues in extended bending regions with an H-bond between its main chain and ligands ILE190 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT