UDP-glucose 4-epimerase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 2udp(A) : MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGK 
         :                                                    
 1lrk(A) : MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGK 
           __________________________________________________ 

           __________________________________________________ 
 2udp(A) : HPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNN 
         :                                                    
 1lrk(A) : HPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNN 
           __________________________________________________ 

           __________________________________________________ 
 2udp(A) : VNGTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYG 
         :                                                    
 1lrk(A) : VNGTLRLISAMRAANVKNFIFSSSATVYGDNPKIPYVESFPTGTPQSPYG 
           __________________________________________________ 

           __________________________________________________ 
 2udp(A) : KSKLMVEQILTDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNL 
         :                                                    
 1lrk(A) : KSKLMVEQILTDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNL 
           __________________________________________________ 

           ___________214_____________230____________________ 
 2udp(A) : MPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKL 
         :                                                    
 1lrk(A) : MPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKL 
           ___________214_____________230____________________ 

           ____________265____272_276_____________________300 
 2udp(A) : ANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW 
         :                                                    
 1lrk(A) : ANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPACW 
           ____________265____272_276_____________________300 

           ______________________________________             
 2udp(A) : ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD             
         :                                                    
 1lrk(A) : ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD             
           ______________________________________             

Details

  Property   Value
 Conformer 1 2udp(A)
 Conformer 2 1lrk(A)
 Radius gyration for conformer 1 19.14 A
 Radius gyration for conformer 2 19.42 A
 EC Number 5.1.3.2  
Click here to see the DynDom results or the famliy
 Trigger ligands UD1341
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA216  ARG231  ARG292  ASN179  ASN198  ASN199  ASP295  GLY229  ILE217  LEU200  LEU215  PHE178  PHE218  THR126  TYR233  VAL269  VAL86 
 Residues in extended bending regions contacting ligands ALA216  ARG231  GLY229  ILE217  LEU215  PHE218  TYR233  VAL269 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA216  ARG231  PHE218 
  Residues in extended bending regions with an H-bond between its main chain and ligands ALA216  PHE218 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT