PROTEIN (UREASE ALPHA SUBUNIT)
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   3ubp(C) : MKINRQQYAESYGPTVGDEVRLADTDLWIEVEKDYTTYGDEVNFGGGKVL           :                                                      4ubp(C) : MKINRQQYAESYGPTVGDEVRLADTDLWIEVEKDYTTYGDEVNFGGGKVL             __________________________________________________             __________________________________________________   3ubp(C) : REGMGENGTYTRTENVLDLLLTNALILDYTGIYKADIGVKDGYIVGIGKG           :                                                      4ubp(C) : REGMGENGTYTRTENVLDLLLTNALILDYTGIYKADIGVKDGYIVGIGKG             __________________________________________________             __________________________________________________   3ubp(C) : GNPDIMDGVTPNMIVGTATEVIAAEGKIVTAGGIDTHVHFINPDQVDVAL           :                                                      4ubp(C) : GNPDIMDGVTPNMIVGTATEVIAAEGKIVTAGGIDTHVHFINPDQVDVAL             __________________________________________________             __________________________________________________   3ubp(C) : ANGITTLFGGGTGPAEGSKATTVTPGPWNIEKMLKSTEGLPINVGILGKG           :                                                      4ubp(C) : ANGITTLFGGGTGPAEGSKATTVTPGPWNIEKMLKSTEGLPINVGILGKG             __________________________________________________             __________________________________________________   3ubp(C) : HGSSIAPIMEQIDAGAAGLiHEDWGATPASIDRSLTVADEADVQVAIHSD           :                                                      4ubp(C) : HGSSIAPIMEQIDAGAAGLiHEDWGATPASIDRSLTVADEADVQVAIHSD             __________________________________________________             __________________________________________________   3ubp(C) : TLNEAGFLEDTLRAINGRVIHSFHVEGAGGGHAPDIMAMAGHPNVLPSST           :                                                      4ubp(C) : TLNEAGFLEDTLRAINGRVIHSFHVEGAGGGHAPDIMAMAGHPNVLPSST             __________________________________________________             _____309__________________________338_____________   3ubp(C) : NPTRPFTVNTIDEHLDMLMVCHHLKQNIPEDVAFADSRIRPETIAAEDIL           :                                                      4ubp(C) : NPTRPFTVNTIDEHLDMLMVCHHLKQNIPEDVAFADSRIRPETIAAEDIL             _____309__________________________338_____________             __________________________________________________   3ubp(C) : HDLGIISMMSTDALAMGRAGEMVLRTWQTADKMKKQRGPLAEEKNGSDNF           :                                                      4ubp(C) : HDLGIISMMSTDALAMGRAGEMVLRTWQTADKMKKQRGPLAEEKNGSDNF             __________________________________________________             __________________________________________________   3ubp(C) : RLKRYVSKYTINPAIAQGIAHEVGSIEEGKFADLVLWEPKFFGVKADRVI           :                                                      4ubp(C) : RLKRYVSKYTINPAIAQGIAHEVGSIEEGKFADLVLWEPKFFGVKADRVI             __________________________________________________             __________________________________________________   3ubp(C) : KGGIIAYAQIGDPSASIPTPQPVMGRRMYGTVGDLIHDTNITFMSKSSIQ           :                                                      4ubp(C) : KGGIIAYAQIGDPSASIPTPQPVMGRRMYGTVGDLIHDTNITFMSKSSIQ             __________________________________________________             __________________________________________________   3ubp(C) : QGVPAKLGLKRRIGTVKNCRNIGKKDMKWNDVTTDIDINPETYEVKVDGE           :                                                      4ubp(C) : QGVPAKLGLKRRIGTVKNCRNIGKKDMKWNDVTTDIDINPETYEVKVDGE             __________________________________________________             ___________________                                  3ubp(C) : VLTCEPVKELPMAQRYFLF                                          :                                                      4ubp(C) : VLTCEPVKELPMAQRYFLF                                            ___________________

Details

Property   Value  Conformer 1 3ubp(C)  Conformer 2 4ubp(C)  Radius gyration for conformer 1 23.65 A  Radius gyration for conformer 2 23.77 A  EC Number 3.5.1.5   Click here to see the DynDom results or the famliy  Trigger ligands HAE800  Trigger ligands in which conformation Conformation  2  Conformaton with trigger ligands is compact No  Spanning ligands No  Residues in enzyme contacting ligands ALA170  ASP363  GLY280  HIS137  HIS139  HIS222  HIS249  HIS275   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT