ASPARTATE AMINOTRANSFERASE
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _______________________________35_________________ 
 1akb(A) : SSWWSHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLNC 
         :                                                    
 7aat(B) : SSWWSHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLNC 
           _______________________________35_________________ 

           __________________________________________________ 
 1akb(A) : VRKAEAMIAAKKMDKEYLPIAGLADFTRASAELALGENSEAFKSGRYVTV 
         :                                                    
 7aat(B) : VRKAEAMIAAKKMDKEYLPIAGLADFTRASAELALGENSEAFKSGRYVTV 
           __________________________________________________ 

           __________________________________________________ 
 1akb(A) : QGISGTGSLRVGANFLQRFFKFSRDVYLPKPSWGNHTPIFRDAGLQLQAY 
         :                                                    
 7aat(B) : QGISGTGSLRVGANFLQRFFKFSRDVYLPKPSWGNHTPIFRDAGLQLQAY 
           __________________________________________________ 

           __________________________________________________ 
 1akb(A) : RYYDPKTCSLDFTGAMEDISKIPEKSIILLHACAHNPTGVDPRQEQWKEL 
         :                                                    
 7aat(B) : RYYDPKTCSLDFTGAMEDISKIPEKSIILLHACAHNPTGVDPRQEQWKEL 
           __________________________________________________ 

           __________________________________________________ 
 1akb(A) : ASVVKKRNLLAYFDMAYQGFASGDINRDAWALRHFIEQGIDVVLSQSYAH 
         :                                                    
 7aat(B) : ASVVKKRNLLAYFDMAYQGFASGDINRDAWALRHFIEQGIDVVLSQSYAK 
           __________________________________________________ 

           __________________________________________________ 
 1akb(A) : NMGLYGERAGAFTVICRDAEEAKRVESQLKILIRPMYSNPPMNGARIASL 
         :                                                    
 7aat(B) : NMGLYGERAGAFTVICRDAEEAKRVESQLKILIRPMYSNPPMNGARIASL 
           __________________________________________________ 

           ________________327___________________________357_ 
 1akb(A) : ILNTPELRKEWLVEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIG 
         :                                                    
 7aat(B) : ILNTPELRKEWLVEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIG 
           ________________327___________________________357_ 

           __________________________________________________ 
 1akb(A) : MFCFTGLKPEQVERLTKEFSIYMTKDGRISVAGVASSNVGYLAHAIHQVT 
         :                                                    
 7aat(B) : MFCFTGLKPEQVERLTKEFSIYMTKDGRISVAGVASSNVGYLAHAIHQVT 
           __________________________________________________ 

           _                                                  
 1akb(A) : K                                                  
         :                                                    
 7aat(B) : K                                                  
           _                                                  

Details

  Property   Value
 Conformer 1 1akb(A)
 Conformer 2 7aat(B)
 Radius gyration for conformer 1 21.93 A
 Radius gyration for conformer 2 22.09 A
 EC Number 2.6.1.1  
Click here to see the DynDom results or the famliy
 Trigger ligands PLP258
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands No
 Residues in enzyme contacting ligands ALA224  ALA257  ARG266  ASN194  ASP222  GLY108  LEU112  LYS258  SER107  SER255  THR109  TRP140  TYR225 
 Residues in extended bending regions contacting ligands Null
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT