Phosphate acetyltransferase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           _____7____________________________________________ 
 1qzt(B) : VTFLEKISERAKKLNKTIALPETEDIRTLQAAAKILERGIADIVLVGNEA 
         :                                                    
 2af3(C) : VTFLEKISERAKKLNKTIALPETEDIRTLQAAAKILERGIADIVLVGNEA 
           _____7____________________________________________ 

           __________________________________________________ 
 1qzt(B) : DIKALAGDLDLSKAKIVDPKTYEKKDEYINAFYELRKHKGITLENAAEIM 
         :                                                    
 2af3(C) : DIKALAGDLDLSKAKIVDPKTYEKKDEYINAFYELRKHKGITLENAAEIM 
           __________________________________________________ 

           ______________________________134_________________ 
 1qzt(B) : SDYVYFAVMMAKLGEVDGVVSGAAHSSSDTLRPAVQIVKTAKGAALASAF 
         :                                                    
 2af3(C) : SDYVYFAVMMAKLGEVDGVVSGAAHSSSDTLRPAVQIVKTAKGAALASAF 
           ______________________________134_________________ 

           __________________________________________________ 
 1qzt(B) : FIISVPDCEYGSDGTFLFADSGMVEMPSVEDVANIAVISAKTFELLVQDV 
         :                                                    
 2af3(C) : FIISVPDCEYGSDGTFLFADSGMVEMPSVEDVANIAVISAKTFELLVQDV 
           __________________________________________________ 

           __________________________________________________ 
 1qzt(B) : PKVAMLSYSTKGSAKSKLTEATIASTKLAQELAPDIAIDGELQVDAAIVP 
         :                                                    
 2af3(C) : PKVAMLSYSTKGSAKSKLTEATIASTKLAQELAPDIAIDGELQVDAAIVP 
           __________________________________________________ 

           ____________________________________________298___ 
 1qzt(B) : KVAASKAPGSPVAGKANVFIFPDLNCGNIAYKIAQRLAKAEAYGPITQGL 
         :                                                    
 2af3(C) : KVAASKAPGSPVAGKANVFIFPDLNCGNIAYKIAQRLAKAEAYGPITQGL 
           ____________________________________________298___ 

           ________________________________                   
 1qzt(B) : AKPINDLSRGCSDEDIVGAVAITCVQAAAQD*                   
         :                                                    
 2af3(C) : AKPINDLSRGCSDEDIVGAVAITCVQAAAQDK                   
           ________________________________                   

Details

  Property   Value
 Conformer 1 1qzt(B)
 Conformer 2 2af3(C)
 Radius gyration for conformer 1 20.76 A
 Radius gyration for conformer 2 21.07 A
 EC Number 2.3.1.8  
Click here to see the DynDom results or the famliy
 Trigger ligands COA501
COA502
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact No
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA148  ALA150  ALA293  ARG87  ASN279  ASP307  CYS312  GLN137  GLN286  GLU176  GLY173  GLY295  LEU132  LEU147  LEU308  LYS283  LYS90  MET174  PHE152  PRO296  SER128  SER309  THR141  THR298  TYR282  TYR294  VAL136  VAL175 
 Residues in extended bending regions contacting ligands ALA148  ALA150  GLN137  GLY295  LEU132  LEU147  PRO296  THR141  THR298  VAL136 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ALA148  GLY295  THR298 
  Residues in extended bending regions with an H-bond between its main chain and ligands ALA148  GLY295 
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT