Tryptophanyl-tRNA synthetase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           ___4____9________________________35_______________ 
 1mau(A) : MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVWQD 
         :                                                    
 1maw(B) : MKTIFSGIQPSGVITIGNYIGALRQFVELQHEYNCYFCIVDQHAITVWQD 
           ___4____9________________________35_______________ 

           _________61__65_______74__________________________ 
 1mau(A) : PHELRQNIRRLAALYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIG 
         :                                                    
 1maw(B) : PHELRQNIRRLAALYLAVGIDPTQATLFIQSEVPAHAQAAWMLQCIVYIG 
           _________61__65_______74__________________________ 

           __________________________________________________ 
 1mau(A) : ELERMTQFKEKSAGKEAVSAGLLTYPPLMAADILLYNTDIVPVGEDQKQH 
         :                                                    
 1maw(B) : ELERMTQFKEKSAGKEAVSAGLLTYPPLMAADILLYNTDIVPVGEDQKQH 
           __________________________________________________ 

           _____________________174__________________________ 
 1mau(A) : IELTRDLAERFNKRYGELFTIPEARIPKVGARIMSLVDPTKKMSKSDPNP 
         :                                                    
 1maw(B) : IELTRDLAERFNKRYGELFTIPEARIPKVGARIMSLVDPTKKMSKSDPNP 
           _____________________174__________________________ 

           __________________________________________________ 
 1mau(A) : KAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISNLLNIYSTLSG 
         :                                                    
 1maw(B) : KAYITLLDDAKTIEKKIKSAVTDSEGTIRYDKEAKPGISNLLNIYSTLSG 
           __________________________________________________ 

           _____________________________________290__________ 
 1mau(A) : QSIEELERQYEGKGYGVFKADLAQVVIETLRPIQERYHHWMESEELDRVL 
         :                                                    
 1maw(B) : QSIEELERQYEGKGYGVFKADLAQVVIETLRPIQERYHHWMESEELDRVL 
           _____________________________________290__________ 

           ____________________________                       
 1mau(A) : DEGAEKANRVASEMVRKMEQAMGLGRRR                       
         :                                                    
 1maw(B) : DEGAEKANRVASEMVRKMEQAMGLGR**                       
           ____________________________                       

Details

  Property   Value
 Conformer 1 1mau(A)
 Conformer 2 1maw(B)
 Radius gyration for conformer 1 20.82 A
 Radius gyration for conformer 2 22.06 A
 EC Number 6.1.1.2  
Click here to see the DynDom results or the famliy
 Trigger ligands LTN390
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ASP132  GLN107  GLN147  GLN9  GLY7  HIS43  ILE133  MET129  PHE5  SER6  TYR125  VAL141  VAL40 
 Residues in extended bending regions contacting ligands GLN9  GLY7  PHE5  SER6 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands Null
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT