Isocitrate dehydrogenase
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1xkd(B) : *SPPCTTEELSPPPGGSLVEYSGGSLRVPDNPVVAFIRGDGVGPEVVESA 
         :                                                    
 1xkd(A) : ASPPCTTEELSPPPGGSLVEYSGGSLRVPDNPVVAFIRGDGVGPEVVESA 
           __________________________________________________ 

           __________________________________________________ 
 1xkd(B) : LKVVDAAVKKV--gSRRIVWWELLAGHLAREKCGELLPKATLEGIRLARV 
         :                                                    
 1xkd(A) : LKVVDAAVKKVYGGSRRIVWWELLAGHLAREKCGELLPKATLEGIRLARV 
           __________________________________________________ 

           __________________________133_____________________ 
 1xkd(B) : ALKGPLETPVGTGYRSLNVAIRQALDLYANIRPVRYYGQPAPHKYADRVD 
         :                                                    
 1xkd(A) : ALKGPLETPV----rSLNVAIRQALDLYANIRPVRYYGQPAPHKYADRVD 
           __________________________133_____________________ 

           __________________________________________________ 
 1xkd(B) : MVIFRENTEDVYAGIEWPHDSPEAARIRRFLAEEFGISIREDAGIGVKPI 
         :                                                    
 1xkd(A) : MVIFRENTEDVYAGIEWPHDSPEAARIRRFLAEEFGISIREDAGIGVKPI 
           __________________________________________________ 

           __________________________________________________ 
 1xkd(B) : SRFATRRLMERALEWALRNGNTVVTIMHKGNIMKYTEGAFMRWAYEVALE 
         :                                                    
 1xkd(A) : SRFATRRLMERALEWALRNGNTVVTIMHKGNIMKYTEGAFMRWAYEVALE 
           __________________________________________________ 

           __________________________________________________ 
 1xkd(B) : KFREHVVTEQEVQEKYGGVRPEGKILVNDRIADNMLQQIITRPWDYQVIV 
         :                                                    
 1xkd(A) : KFREHVVTEQEVQEKYGGVRPEGKILVNDRIADNMLQQIITRPWDYQVIV 
           __________________________________________________ 

           ________________________331_______________________ 
 1xkd(B) : APNLNGDYISDAASALVGGIGMAAGMNMGDGIAVAEPVHGTAPKYAGKDL 
         :                                                    
 1xkd(A) : APNLNGDYISDAASALVGGIGMAAGMNMGDGIAVAEPVHGTAPKYAGKDL 
           ________________________331_______________________ 

           ____________________________________________401___ 
 1xkd(B) : INPSAEILSASLLIGEFMGWREVKSIVEYAIRKAVQSKKVTQDLARHMPG 
         :                                                    
 1xkd(A) : INPSAEILSASLLIGEFMGWREVKSIVEYAIRKAVQSKKVTQDLARHMPG 
           ____________________________________________401___ 

           _______________________________                    
 1xkd(B) : VQPLRTSEYTETLIAYIDEADL*********                    
         :                                                    
 1xkd(A) : VQPLRTSEYTETLIAYIDEADLNEVLAGKRG                    
           _______________________________                    

Details

  Property   Value
 Conformer 1 1xkd(B)
 Conformer 2 1xkd(A)
 Radius gyration for conformer 1 22.57 A
 Radius gyration for conformer 2 22.79 A
 EC Number 1.1.1.42  
Click here to see the DynDom results or the famliy
 Trigger ligands ICI1004
 Trigger ligands in which conformation Conformation  1
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ARG126  ARG136  ARG159  ASN122  ASP311  SER120  THR112  TYR166  VAL123  VAL342 
 Residues in extended bending regions contacting ligands ARG136 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands ARG136 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT