long chain fatty acid-CoA ligase
Ligand-induced domain movement details

Jmol view of liganded conformation 2

Domain Movement

domain 1 is binding domain(fixed in space) domain 2 is binding domain(fixed in space)

Sequence


           __________________________________________________ 
 1ult(A) : AFPSTMMDEELNLWDFLERAAALFGRKEVVSRLHTGEVHRTTYAEVYQRA 
         :                                                    
 1v25(B) : AFPSTMMDEELNLWDFLERAAALFGRKEVVSRLHTGEVHRTTYAEVYQRA 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : RRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLS 
         :                                                    
 1v25(B) : RRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLS 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : PKEIAYILNHAEDKVLLFDPNLLPLVEAIRGELKTVQHFVVMDEKAPEGY 
         :                                                    
 1v25(B) : PKEIAYILNHAEDKVLLFDPNLLPLVEAIRGELKTVQHFVVMDEKAPEGY 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : LAYEEALGEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLA 
         :                                                    
 1v25(B) : LAYEEALGEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLA 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : ASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPA 
         :                                                    
 1v25(B) : ASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGPRLDPA 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : SLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPR 
         :                                                    
 1v25(B) : SLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPR 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : SLIARFERMGVEVRQGYGLTETSPVVVQNFVKSHLESLSEEEKLTLKAKT 
         :                                                    
 1v25(B) : SLIARFERMGVEVRQGYGLTETSPVVVQNFVKSHLESLSEEEKLTLKAKT 
           __________________________________________________ 

           __________________________________________________ 
 1ult(A) : GLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEATRSA 
         :                                                    
 1v25(B) : GLPIPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEATRSA 
           __________________________________________________ 

           ____________________430___________________________ 
 1ult(A) : LTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGH 
         :                                                    
 1v25(B) : LTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGH 
           ____________________430___________________________ 

           __________________________________________________ 
 1ult(A) : PKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPEELNEHLLKAGFAKWQL 
         :                                                    
 1v25(B) : -----aAVVAIPHPKWQERPLAV-------------nEHLLKAGFAKWQL 
           __________________________________________________ 

           __________________________________                 
 1ult(A) : PDAYVFAEEIPRTSAGKFLKRALREQYKNYYGG*                 
         :                                                    
 1v25(B) : PDAYVF---------gKFLKRALREQYKNYYGGA                 
           __________________________________                 

Details

  Property   Value
 Conformer 1 1ult(A)
 Conformer 2 1v25(B)
 Radius gyration for conformer 1 24.41 A
 Radius gyration for conformer 2 22.73 A
 EC Number 6.2.1.3  
Click here to see the DynDom results or the famliy
 Trigger ligands ANP1666
 Trigger ligands in which conformation Conformation  2
 Conformaton with trigger ligands is compact Yes
 Spanning ligands Yes
 Residues in enzyme contacting ligands ALA304  ARG433  ASP418  GLN322  GLY302  GLY323  GLY325  HIS230  ILE430  LEU326  LYS435  LYS439  PHE229  SER303  THR327  TRP234  TRP444  TYR324  VAL231 
 Residues in extended bending regions contacting ligands ARG433  ILE430  LYS435  LYS439 
 Residues in extended bending regions making H-bonds or salt-bridges with ligands LYS435  LYS439 
  Residues in extended bending regions with an H-bond between its main chain and ligands Null
Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT